|
| Status |
Public on Oct 24, 2016 |
| Title |
KO_Ly6C- F4/80high, biological rep2, non-treated, day 2 |
| Sample type |
RNA |
| |
|
| Source name |
muscle-derived tissue macrophage
|
| Organism |
Mus musculus |
| Characteristics |
strain: KO (C57B6/J, PPARg fl/fl, LysM Cre+)
cell surafce marker: Ly6C- F4/80high
treatment: non-treated
time: day 2
|
| Treatment protocol |
CD45+ hematopoietic cells from regenerating muscles were isolated with a magnetic bead selection (Miltenyi). Cells were labeled with PE-conjugated Ly6C and APC-conjugated F4/80 antibodies and then sorted into Ly6C+ F4/80low and Ly6C- F4/80high subsets.
|
| Growth protocol |
Muscle injury was caused by cardiotoxin ijection into the tibialis anterior muscle. Macrophages were separated after 1, 2 and 4 days post-injury from the regenerating muscle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Ambion WT Expression Kit (Applied Biosystems) and GeneChip WT Terminal Labeling and Control Kit (Affymetrix) were used for amplifying and labeling 150 ng of RNA samples.
|
| |
|
| Hybridization protocol |
Samples were hybridized at 45 degrees Celsius for 16 hours and then standard washing protocol was performed using GeneChip Fluidics Station 450 (Affymetrix).
|
| Scan protocol |
Arrays were scanned on GeneChip Scanner 7G (Affymetrix), Affymetrix GeneChip Command Console (AGCC) was used to obtain image files.
|
| Description |
Gene expression data of muscle-derived macrophages
|
| Data processing |
CHP files were generated using Affymetrix Expression Control.
Downstream data analysis was performed in GeneSpring GX 12, CEL files were imported using RMA algorithm and median normalization was performed.
|
| |
|
| Submission date |
Jul 21, 2015 |
| Last update date |
Oct 24, 2016 |
| Contact name |
Szilard Poliska |
| E-mail(s) |
poliska@dote.hu
|
| Phone |
+3652416432
|
| Organization name |
University of Debrecen
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Nuclear Hormone Receptor Research Laboratory
|
| Street address |
Egyetem ter 1.
|
| City |
Debrecen |
| ZIP/Postal code |
H-4012 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE71155 |
Identification of a macrophage PPARĪ³-GDF3 regulatory axis controlling muscle regeneration |
|
