|
| Status |
Public on Jul 23, 2015 |
| Title |
C4 13C |
| Sample type |
RNA |
| |
|
| Source name |
stable isotope incubations of Monterey Bay surface water
|
| Organism |
marine metagenome |
| Characteristics |
tissue: surface marine microbes (<1 micron) collected from Monterey Bay seawater
incubation condition: glucose, 36 hours
|
| Treatment protocol |
samples included \ 13C labeled glucose, starch, acetate, lipids, protein, or ammino acids added at 1 micromolar C final concentration
|
| Growth protocol |
1 micron filtered samples incubated in 12 Liter bottles at 20 degrees Celsius for 12 hours or 36 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen Rneasy kit
|
| Label |
13C
|
| Label protocol |
samples 1 and 2 were labeled with Alexafluor 532 with the Ulysis labeling kit from Life Technologies
|
| |
|
| Hybridization protocol |
1 microgram RNA was hybridized with Nimblegen Hybridization buffer at 42 degrees Celsius for 18 hours, and washed with Nimblegen Wash buffers I, II, and III
|
| Scan protocol |
samples 1 and 2 were scanned with a Genepix 4000B at PMT = 800, other samples scanned with a NanoSIMS 50 according to protocol from Mayali et al. (2012) ISME J 6:1210-1221.
|
| Data processing |
Isotope ratios extracted using software package LIMAGE (L. Nittle, Carnegie Institute, Washington DC)
|
| |
|
| Submission date |
Jul 22, 2015 |
| Last update date |
Jul 23, 2015 |
| Contact name |
Xavier Mayali |
| E-mail(s) |
mayali1@llnl.gov
|
| Phone |
925-423-3892
|
| Organization name |
Lawrence Livermore National Laboratory
|
| Department |
Chemical Science
|
| Street address |
7000 East Ave
|
| City |
Livermore |
| State/province |
CA |
| ZIP/Postal code |
94550 |
| Country |
USA |
| |
|
| Platform ID |
GPL20727 |
| Series (1) |
| GSE71228 |
Chip-SIP analysis of Monterey Bay surface waters incubated with organic carbon substrates |
|
