|
| Status |
Public on Oct 01, 2015 |
| Title |
MCF7 Hotair +E2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
genotype/variation: HOTAIR
agent: 1 nM E2
|
| Treatment protocol |
Lentiviral infected Ctrl or HOTAIR stable MCF7 cells were deprived for 3 days and then treated with ethanol or 1 nM E2 for 6h, cells were collected for RNA extraction.
|
| Growth protocol |
Cells were maintained in DMEM supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. The quantity and quality of the isolated RNAs were determined using ND-1000 Spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| Scan protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| Description |
SAMPLE 4
|
| Data processing |
The data were processed and normalised using Illumina GenomeStudio
|
| |
|
| Submission date |
Jul 24, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Jindan Yu |
| E-mail(s) |
jindan.yu@emory.edu
|
| Organization name |
Emory University
|
| Department |
Urology
|
| Lab |
Jindan Yu's lab
|
| Street address |
E330, 1760 Haygood Dr NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE71297 |
LncRNA HOTAIR enhances ER signaling and confers tamoxifen resistance in breast cancer [array] |
| GSE71298 |
LncRNA HOTAIR enhances ER signaling and confers tamoxifen resistance in breast cancer |
|
