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Status |
Public on Jul 16, 2018 |
Title |
6-48 0.5mM OTA_18238 |
Sample type |
RNA |
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Source name |
embryos
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Organism |
Danio rerio |
Characteristics |
developemental stage: 6 hpf embryos exposed to: 0.5 µM ochratoxin A from 6 hpf to 48 hpf
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Treatment protocol |
6 hpf embryos were treated with ochratoxin A (0.5 µM) till 48 hpf.
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Growth protocol |
zebrafish embryos were selected at 6 hph with normal development.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by Trizol® Reagent (Invitrogen, USA) according to the instruction manual. RNA purified was quantified at OD260nm by using a ND-1000 spectrophotometer (Nanodrop Technology, USA) and qualitated by using a Bioanalyzer 2100 (Agilent Technology, USA) with RNA 6000 labchip kit (Agilent Technologies, USA).
|
Label |
Cy3
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Label protocol |
0.2 mg of total RNA was amplified by a low Input Quick-Amp Labeling kit (Agilent Technologies, USA) and labeled with Vy3 (CyDye, Agilent Technologies, USA) during the in vitro transcription process.
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Hybridization protocol |
1.65 mg of Cy3-labled cRNA is then pooled and hybridized to Agilent Zebrafish V3 array 4´44K Microarray (Agilent Technologies, USA) at 65℃ for 17h.
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Scan protocol |
After washing and drying by nitrogen fun blowing, microarrays are scanned with an Agilent microarray scanner (Agilent Technologies, USA) at 535nm for Cy3.
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Data processing |
The scanned images were analyzed with Feature Extraction 10.5.1.1 Software (Agilent Technologies, USA), an image analysis and normalization software is used to quantify signal and background intensity for each feature.
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Submission date |
Jul 25, 2015 |
Last update date |
Jul 16, 2018 |
Contact name |
Ting-Shuan Wu |
E-mail(s) |
ab118tw@gmail.com
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Organization name |
ntu
|
Street address |
5F ,No 1, Sec 1, Jen-Ai Rd
|
City |
Taipei |
ZIP/Postal code |
100 |
Country |
Taiwan |
|
|
Platform ID |
GPL14688 |
Series (1) |
GSE71345 |
Agilent zebrafish V3 array 4´44K microarray-Ochratoxin A treated with 6-48 hpf zebrafish embryos |
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