|
Status |
Public on Sep 19, 2015 |
Title |
Zeb2 KO, replicate 1 |
Sample type |
RNA |
|
|
Source name |
P14 cells sorted at d6 of LCMV infection of Zeb2 KO mice
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 gender: Male age: 6 weeks tissue: Spleen genotype/variation: Zeb2 KO phenotypic marker: CD8+ CD45.1- CD45.2+ CD4- MHCII- B220- Gr1- NK1.1-
|
Treatment protocol |
1X10^4 cells from WT or KO mice were co-transferred into cogenically matched recipient mice. Mice were infected with LCMV or Listeria-OVA the next day. On day6 of infection cells are purified by Facs Aria sorting.
|
Extracted molecule |
total RNA |
Extraction protocol |
Glass slide teasing Flow sorting
|
Label |
biotin
|
Label protocol |
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
|
|
|
Hybridization protocol |
Affy 1.0 ST Hyb Kit
|
Scan protocol |
Affymetrix GeneChip Scanner
|
Description |
P14 cells sorted at d6 of LCMV infection of Zeb2 KO mice
|
Data processing |
RMA, Genepattern, The Sample data table contains raw expression data post RMA, excluding unlabeled probes or duplicate probes (that showed identical value).
|
|
|
Submission date |
Aug 18, 2015 |
Last update date |
Sep 19, 2015 |
Contact name |
Howard Y Chang |
E-mail(s) |
howchang@stanford.edu
|
Organization name |
Stanford University
|
Street address |
269 Campus Drive
|
City |
Stanford |
State/province |
California |
ZIP/Postal code |
94305-5168 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE72162 |
Gene expression data from Zeb2WT, Zeb2KO, T-betWT and T-betKO effector CD8+ T cells during infection |
|