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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 19, 2015 |
| Title |
Gene expression data from Zeb2WT, Zeb2KO, T-betWT and T-betKO effector CD8+ T cells during infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
ZEB2 is a multi-zinc-finger transcription factor known to play a significant role in early neurogenesis and in EMT-dependent tumor metastasis. While the function of ZEB2 in T lymphocytes is unknown, activity of the closely related family member ZEB1 has been implicated in lymphocyte development. Here, we find that ZEB2 expression is upregulated by activated T cells, specifically in the KLRG1hi effector CD8+ T cell subset. Loss of ZEB2 expression results in a significant loss of antigen-specific CD8+ T cells following primary and secondary infection with a severe impairment in the generation of the KLRG1hi effector-memory cell population. We show that ZEB2, which can bind DNA at tandem, consensus E-box sites, regulates gene expression of several E-protein targets and may directly repress CD127 and IL-2 in CD8+ T cells responding to infection. Furthermore, we find that T-bet binds to highly conserved T-box-sites in the ZEB2 gene and that T-bet and ZEB2 regulate similar gene-expression programs in effector T cells, suggesting that T-bet acts upstream and through regulation of ZEB2. Taken together, we place ZEB2 in a larger transcriptional network that is responsible for the balance between terminal differentiation and formation of memory CD8+ T cells.
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| Overall design |
Zeb2WT, Zeb2KO, T-betWT and T-betKO effector CD8+ T cells were sorted into Trizol at day 6 of infection. Two or more replicates per sample were analyzed
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| Contributor(s) |
Omilusik KD, Best JA, Yu B, Goldrath AW |
| Citation(s) |
26503445 |
| Submission date |
Aug 18, 2015 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Howard Y Chang |
| E-mail(s) |
howchang@stanford.edu
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| Organization name |
Stanford University
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| Street address |
269 Campus Drive
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| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305-5168 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA293220 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72162_RAW.tar |
40.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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