|
| Status |
Public on Sep 19, 2015 |
| Title |
T-bet KO, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
OT1 cells sorted at d6 of Listeria-OVA infection of T-bet KO mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: Male
age: 6 weeks
tissue: Spleen
genotype/variation: T-bet KO
phenotypic marker: CD8+ CD45.1+ CD45.2- CD4- MHCII- B220- Gr1- NK1.1-
|
| Treatment protocol |
1X10^4 cells from WT or KO mice were co-transferred into cogenically matched recipient mice. Mice were infected with LCMV or Listeria-OVA the next day. On day6 of infection cells are purified by Facs Aria sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Glass slide teasing Flow sorting
|
| Label |
biotin
|
| Label protocol |
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
|
| |
|
| Hybridization protocol |
Affy 1.0 ST Hyb Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Description |
OT1 cells sorted at d6 of Listeria-OVA infection of T-bet KO mice
|
| Data processing |
RMA, Genepattern, The Sample data table contains raw expression data post RMA, excluding unlabeled probes or duplicate probes (that showed identical value).
|
| |
|
| Submission date |
Aug 18, 2015 |
| Last update date |
Sep 19, 2015 |
| Contact name |
Howard Y Chang |
| E-mail(s) |
howchang@stanford.edu
|
| Organization name |
Stanford University
|
| Street address |
269 Campus Drive
|
| City |
Stanford |
| State/province |
California |
| ZIP/Postal code |
94305-5168 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE72162 |
Gene expression data from Zeb2WT, Zeb2KO, T-betWT and T-betKO effector CD8+ T cells during infection |
|
