|
| Status |
Public on Feb 10, 2017 |
| Title |
muscle_old_MISR_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
whole skeletal muscle
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 24 months
genotype: MISR
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from muscle tissue using Trizol (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
RNA samples were reverse-transcribed, amplified and biotin-labeled using GeneChip WT PLUS Reagent Kit from Affymetrix
|
| |
|
| Hybridization protocol |
RNA samples were hybridized to Mouse Gene 2.0 ST Arrays (Affymetrix) following the manufacturer's protocol
|
| Scan protocol |
Arrays were scanned by Affymetrix GeneChip Scanner 3000
|
| Data processing |
Arrays were normalized using the robust multi-array average (RMA) algorithm
|
| |
|
| Submission date |
Aug 19, 2015 |
| Last update date |
Feb 10, 2017 |
| Contact name |
Jonathan M Dreyfuss |
| Organization name |
Joslin Diabetes Center
|
| Department |
Bioinformatics & Biostatistics Core
|
| Street address |
1 Joslin Pl
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE72179 |
Increased activity of canonical NF-kB signaling in muscle fibers alters the satellite cell niche and restrains muscle stem cell function during aging |
|
