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Sample GSM1872257 Query DataSets for GSM1872257
Status Public on Sep 15, 2015
Title dicerwt_R1 Replicate 2
Sample type RNA
Source name RKO dicer wild-type cells
Organism Homo sapiens
Characteristics genotype/variation: dicer wt
transgene: cells expressing wild-type RNase1
Growth protocol Cells were cultured in serum-free PC-1 media (Lonza).
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by two additional round of phenol-chloroform extraction.
Label Cy3,Cy5
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA miRNA Hi-Power Labeling kit, Hy3/Hy5 (Exiqon,Denmark) following the procedure described by the manufaturer.
Hybridization protocol The hybridization was performed according to the miRCURY LNA miRNA Array Instruction manual using a Tecan HS4800 hybridization station.
Scan protocol The miRCURY LNA micro RNA Array slides were scanned using the Agilent G2565 BA Microarray Scanner System.
Data processing The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess regression algorithm. The normalized expression levels are expressed in a log2 scale.
Submission date Sep 08, 2015
Last update date Sep 15, 2015
Contact name Emanuele Canestrari
Organization name University of Illinois at Chicago
Street address 900 S. Ashland Ave.(M/C 669)
City Chicago
ZIP/Postal code 60607
Country USA
Platform ID GPL20891
Series (1)
GSE72796 Rnase1 responsive miRNA

Data table header descriptions
VALUE lowess log2 values

Data table
4040 5.600039867
4610 7.44667814
4700 6.560675735
5250 5.476720801
6880 5.859542142
9938 9.904471672
10138 5.799384654
10306 5.587426613
10916 5.601148906
10919 9.96954492
10923 9.049998612
10925 6.713948923
10928 8.592609001
10936 7.449447555
10937 5.776721064
10943 5.601945388
10946 6.25666642
10947 5.600536327
10952 5.399308072
10954 5.629510844

Total number of rows: 2100

Table truncated, full table size 37 Kbytes.

Supplementary file Size Download File type/resource
GSM1872257_Cy3_Exiqon_19735943_S01.txt.gz 1.5 Mb (ftp)(http) TXT
GSM1872257_Cy5_Exiqon_19735943_S01.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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