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Series GSE72796 Query DataSets for GSE72796
Status Public on Sep 15, 2015
Title Rnase1 responsive miRNA
Platform organisms Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Macacine alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Saimiriine gammaherpesvirus 2; JC polyomavirus; Human immunodeficiency virus 1; Human betaherpesvirus 6B; Human gammaherpesvirus 8; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary To determine the importance of RNase1 on cellular miRNA function, we used a lentivirus system to stably express RNase1 in dicer wt and dicer ex5 RKO cells in serum free culture. RNase1 was found to govern miRNA that reside outside the pureview of Dicer. Assignment of RNase1 as miRNA-generating enzyme suggests that, like Dicer, RNaseA enzymes mediate essential physiological functions through regulatory RNA.
Overall design Two-condition experiment, wild-type RNase1 vs mutant (6x) RNase1,in two cell lines RKO dicer wild-type and RKO dicer mutant (ex5). Biological replicates: 2 for each condition and each cell line . One replicate per array.
Contributor(s) Canestrari E, Asada K, Paroo Z
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Submission date Sep 08, 2015
Last update date Sep 19, 2015
Contact name Emanuele Canestrari
Organization name University of Illinois at Chicago
Street address 900 S. Ashland Ave.(M/C 669)
City Chicago
ZIP/Postal code 60607
Country USA
Platforms (1)
GPL20891 Exiqon, miRCURY LNA microRNA array, 7th generation (hsa, mirbase 21)
Samples (8)
GSM1872256 dicerwt_R1 Replicate 1
GSM1872257 dicerwt_R1 Replicate 2
GSM1872258 dicerwt_6x Replicate 1
BioProject PRJNA295979

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Supplementary file Size Download File type/resource
GSE72796_RAW.tar 23.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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