|
| Status |
Public on Sep 15, 2015 |
| Title |
Rnase1 responsive miRNA |
| Platform organisms |
Homo sapiens; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Macacine alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Saimiriine gammaherpesvirus 2; JC polyomavirus; Human immunodeficiency virus 1; Human betaherpesvirus 6B; Human gammaherpesvirus 8; Merkel cell polyomavirus; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
To determine the importance of RNase1 on cellular miRNA function, we used a lentivirus system to stably express RNase1 in dicer wt and dicer ex5 RKO cells in serum free culture. RNase1 was found to govern miRNA that reside outside the pureview of Dicer. Assignment of RNase1 as miRNA-generating enzyme suggests that, like Dicer, RNaseA enzymes mediate essential physiological functions through regulatory RNA.
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| Overall design |
Two-condition experiment, wild-type RNase1 vs mutant (6x) RNase1,in two cell lines RKO dicer wild-type and RKO dicer mutant (ex5). Biological replicates: 2 for each condition and each cell line . One replicate per array.
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| Contributor(s) |
Canestrari E, Asada K, Paroo Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 08, 2015 |
| Last update date |
Sep 19, 2015 |
| Contact name |
Emanuele Canestrari |
| E-mail(s) |
manucns@uic.edu
|
| Organization name |
University of Illinois at Chicago
|
| Street address |
900 S. Ashland Ave.(M/C 669)
|
| City |
Chicago |
| ZIP/Postal code |
60607 |
| Country |
USA |
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| Platforms (1) |
| GPL20891 |
Exiqon, miRCURY LNA microRNA array, 7th generation (hsa, mirbase 21) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA295979 |
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