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Sample GSM1872258 Query DataSets for GSM1872258
Status Public on Sep 15, 2015
Title dicerwt_6x Replicate 1
Sample type RNA
Source name RKO dicer wild-type cells
Organism Homo sapiens
Characteristics genotype/variation: dicer wt
transgene: cells expressing mutant RNase1
Growth protocol Cells were cultured in serum-free PC-1 media (Lonza).
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by two additional round of phenol-chloroform extraction.
Label Cy3,Cy5
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA miRNA Hi-Power Labeling kit, Hy3/Hy5 (Exiqon,Denmark) following the procedure described by the manufaturer.
Hybridization protocol The hybridization was performed according to the miRCURY LNA miRNA Array Instruction manual using a Tecan HS4800 hybridization station.
Scan protocol The miRCURY LNA micro RNA Array slides were scanned using the Agilent G2565 BA Microarray Scanner System.
Data processing The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess regression algorithm. The normalized expression levels are expressed in a log2 scale.
Submission date Sep 08, 2015
Last update date Sep 15, 2015
Contact name Emanuele Canestrari
Organization name University of Illinois at Chicago
Street address 900 S. Ashland Ave.(M/C 669)
City Chicago
ZIP/Postal code 60607
Country USA
Platform ID GPL20891
Series (1)
GSE72796 Rnase1 responsive miRNA

Data table header descriptions
VALUE lowess log2 values

Data table
4040 5.110387246
4610 7.538428963
4700 6.510187082
5250 4.859819889
6880 5.669716716
9938 9.992604531
10138 5.573479096
10306 5.079765395
10916 5.150928162
10919 10.09911666
10923 9.1924378
10925 6.767531244
10928 8.720500621
10936 7.562286103
10937 5.305577512
10943 5.216618979
10946 6.177767779
10947 5.176734293
10952 4.876429534
10954 5.20057328

Total number of rows: 2100

Table truncated, full table size 37 Kbytes.

Supplementary file Size Download File type/resource
GSM1872258_Cy3_Exiqon_19735941_S01.txt.gz 1.5 Mb (ftp)(http) TXT
GSM1872258_Cy5_Exiqon_19735941_S01.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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