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Sample GSM1872260 Query DataSets for GSM1872260
Status Public on Sep 15, 2015
Title ex5_R1 Replicate 1
Sample type RNA
Source name RKO dicer hypomorph (ex5) cells
Organism Homo sapiens
Characteristics genotype/variation: dicer ex5 RKO
transgene: cells expressing wild-type RNase1
Growth protocol Cells were cultured in serum-free PC-1 media (Lonza).
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by two additional round of phenol-chloroform extraction.
Label Cy3,Cy5
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA miRNA Hi-Power Labeling kit, Hy3/Hy5 (Exiqon,Denmark) following the procedure described by the manufaturer.
Hybridization protocol The hybridization was performed according to the miRCURY LNA miRNA Array Instruction manual using a Tecan HS4800 hybridization station.
Scan protocol The miRCURY LNA micro RNA Array slides were scanned using the Agilent G2565 BA Microarray Scanner System.
Data processing The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess regression algorithm. The normalized expression levels are expressed in a log2 scale.
Submission date Sep 08, 2015
Last update date Sep 15, 2015
Contact name Emanuele Canestrari
Organization name University of Illinois at Chicago
Street address 900 S. Ashland Ave.(M/C 669)
City Chicago
ZIP/Postal code 60607
Country USA
Platform ID GPL20891
Series (1)
GSE72796 Rnase1 responsive miRNA

Data table header descriptions
VALUE lowess log2 values

Data table
4040 5.027506537
4610 7.569870758
4700 6.514312613
5250 4.689496917
6880 5.608310084
9938 10.14208024
10138 5.373874737
10306 4.920553349
10916 4.905600463
10919 10.07628831
10923 9.33879397
10925 6.801861649
10928 8.904335668
10936 7.643573247
10937 5.272487571
10943 5.006909056
10946 6.028652354
10947 4.921462122
10952 4.706612098
10954 5.142794067

Total number of rows: 2100

Table truncated, full table size 37 Kbytes.

Supplementary file Size Download File type/resource
GSM1872260_Cy3_Exiqon_19735991_S01.txt.gz 1.5 Mb (ftp)(http) TXT
GSM1872260_Cy5_Exiqon_19735991_S01.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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