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Sample GSM1872261 Query DataSets for GSM1872261
Status Public on Sep 15, 2015
Title ex5_R1 Replicate 2
Sample type RNA
Source name RKO dicer hypomorph (ex5) cells
Organism Homo sapiens
Characteristics genotype/variation: dicer ex5 RKO
transgene: cells expressing wild-type RNase1
Growth protocol Cells were cultured in serum-free PC-1 media (Lonza).
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by two additional round of phenol-chloroform extraction.
Label Cy3,Cy5
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA miRNA Hi-Power Labeling kit, Hy3/Hy5 (Exiqon,Denmark) following the procedure described by the manufaturer.
Hybridization protocol The hybridization was performed according to the miRCURY LNA miRNA Array Instruction manual using a Tecan HS4800 hybridization station.
Scan protocol The miRCURY LNA micro RNA Array slides were scanned using the Agilent G2565 BA Microarray Scanner System.
Data processing The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess regression algorithm. The normalized expression levels are expressed in a log2 scale.
Submission date Sep 08, 2015
Last update date Sep 15, 2015
Contact name Emanuele Canestrari
Organization name University of Illinois at Chicago
Street address 900 S. Ashland Ave.(M/C 669)
City Chicago
ZIP/Postal code 60607
Country USA
Platform ID GPL20891
Series (1)
GSE72796 Rnase1 responsive miRNA

Data table header descriptions
VALUE lowess log2 values

Data table
4040 5.273410241
4610 7.436560275
4700 6.50165653
5250 5.047561732
6880 5.723802365
9938 10.06377973
10138 5.391514082
10306 5.238182443
10916 5.285689169
10919 10.10542179
10923 9.289860364
10925 6.779791006
10928 8.796149713
10936 7.567861216
10937 5.511372085
10943 5.211876906
10946 6.077728506
10947 5.374084717
10952 5.162999632
10954 5.323888865

Total number of rows: 2100

Table truncated, full table size 37 Kbytes.

Supplementary file Size Download File type/resource
GSM1872261_Cy3_Exiqon_19735944_S01.txt.gz 1.5 Mb (ftp)(http) TXT
GSM1872261_Cy5_Exiqon_19735944_S01.txt.gz 1.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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