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Sample GSM1872262 Query DataSets for GSM1872262
Status Public on Sep 15, 2015
Title ex5_6x Replicate 1
Sample type RNA
Source name RKO dicer hypomorph (ex5) cells
Organism Homo sapiens
Characteristics genotype/variation: dicer ex5 RKO
transgene: cells expressing mutant RNase1
Growth protocol Cells were cultured in serum-free PC-1 media (Lonza).
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by two additional round of phenol-chloroform extraction.
Label Cy3,Cy5
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA miRNA Hi-Power Labeling kit, Hy3/Hy5 (Exiqon,Denmark) following the procedure described by the manufaturer.
Hybridization protocol The hybridization was performed according to the miRCURY LNA miRNA Array Instruction manual using a Tecan HS4800 hybridization station.
Scan protocol The miRCURY LNA micro RNA Array slides were scanned using the Agilent G2565 BA Microarray Scanner System.
Data processing The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess regression algorithm. The normalized expression levels are expressed in a log2 scale.
Submission date Sep 08, 2015
Last update date Sep 15, 2015
Contact name Emanuele Canestrari
Organization name University of Illinois at Chicago
Street address 900 S. Ashland Ave.(M/C 669)
City Chicago
ZIP/Postal code 60607
Country USA
Platform ID GPL20891
Series (1)
GSE72796 Rnase1 responsive miRNA

Data table header descriptions
VALUE lowess log2 values

Data table
4040 4.945716065
4610 7.271138324
4700 6.298001786
5250 5.014505504
6880 5.594734941
9938 9.822006685
10138 5.187721919
10306 5.031339956
10916 5.030542936
10919 9.938128366
10923 9.09691854
10925 6.628328375
10928 8.564438052
10936 7.353750986
10937 5.148655388
10943 5.036891977
10946 5.909784742
10947 5.119325547
10952 4.889008377
10954 5.050128389

Total number of rows: 2100

Table truncated, full table size 38 Kbytes.

Supplementary file Size Download File type/resource
GSM1872262_Cy3_Exiqon_19735989_S01.txt.gz 1.5 Mb (ftp)(http) TXT
GSM1872262_Cy5_Exiqon_19735989_S01.txt.gz 1.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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