|
| Status |
Public on Sep 15, 2015 |
| Title |
ex5_6x Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
RKO dicer hypomorph (ex5) cells
|
| Organism |
Homo sapiens |
| Characteristics |
genotype/variation: dicer ex5 RKO
transgene: cells expressing mutant RNase1
|
| Growth protocol |
Cells were cultured in serum-free PC-1 media (Lonza).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol followed by two additional round of phenol-chloroform extraction.
|
| Label |
Cy3,Cy5
|
| Label protocol |
750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA miRNA Hi-Power Labeling kit, Hy3/Hy5 (Exiqon,Denmark) following the procedure described by the manufaturer.
|
| |
|
| Hybridization protocol |
The hybridization was performed according to the miRCURY LNA miRNA Array Instruction manual using a Tecan HS4800 hybridization station.
|
| Scan protocol |
The miRCURY LNA micro RNA Array slides were scanned using the Agilent G2565 BA Microarray Scanner System.
|
| Data processing |
The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess regression algorithm. The normalized expression levels are expressed in a log2 scale.
|
| |
|
| Submission date |
Sep 08, 2015 |
| Last update date |
Sep 15, 2015 |
| Contact name |
Emanuele Canestrari |
| E-mail(s) |
manucns@uic.edu
|
| Organization name |
University of Illinois at Chicago
|
| Street address |
900 S. Ashland Ave.(M/C 669)
|
| City |
Chicago |
| ZIP/Postal code |
60607 |
| Country |
USA |
| |
|
| Platform ID |
GPL20891 |
| Series (1) |
|
