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Sample GSM1872263 Query DataSets for GSM1872263
Status Public on Sep 15, 2015
Title ex5_6x Replicate 2
Sample type RNA
Source name RKO dicer hypomorph (ex5) cells
Organism Homo sapiens
Characteristics genotype/variation: dicer ex5 RKO
transgene: cells expressing mutant RNase1
Growth protocol Cells were cultured in serum-free PC-1 media (Lonza).
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol followed by two additional round of phenol-chloroform extraction.
Label Cy3,Cy5
Label protocol 750 ng total RNA from both sample and reference was labeled with Hy3 and Hy5 fluorescent label, respectively, using the miRCURY LNA miRNA Hi-Power Labeling kit, Hy3/Hy5 (Exiqon,Denmark) following the procedure described by the manufaturer.
Hybridization protocol The hybridization was performed according to the miRCURY LNA miRNA Array Instruction manual using a Tecan HS4800 hybridization station.
Scan protocol The miRCURY LNA micro RNA Array slides were scanned using the Agilent G2565 BA Microarray Scanner System.
Data processing The quantified signals were background corrected (Normexp with offset value 10) and normalized using the global Lowess regression algorithm. The normalized expression levels are expressed in a log2 scale.
Submission date Sep 08, 2015
Last update date Sep 15, 2015
Contact name Emanuele Canestrari
Organization name University of Illinois at Chicago
Street address 900 S. Ashland Ave.(M/C 669)
City Chicago
ZIP/Postal code 60607
Country USA
Platform ID GPL20891
Series (1)
GSE72796 Rnase1 responsive miRNA

Data table header descriptions
VALUE lowess log2 values

Data table
4040 4.69148703
4610 7.570306494
4700 6.426119482
5250 4.606019781
6880 5.626172612
9938 10.16402234
10138 5.177605706
10306 4.794768218
10916 4.679638554
10919 10.1965232
10923 9.350587895
10925 6.821376019
10928 8.903430294
10936 7.640635934
10937 5.208799369
10943 4.79855284
10946 6.007159575
10947 4.792035109
10952 4.534114872
10954 5.16047172

Total number of rows: 2100

Table truncated, full table size 37 Kbytes.

Supplementary file Size Download File type/resource
GSM1872263_Cy3_Exiqon_19735990_S01.txt.gz 1.5 Mb (ftp)(http) TXT
GSM1872263_Cy5_Exiqon_19735990_S01.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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