|
| Status |
Public on Mar 14, 2018 |
| Title |
Mesocosm growth, day 7, Replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mycelia, drought, day 7
|
| Organism |
Agaricus bisporus |
| Characteristics |
strain: var bisporus (H97)
tissue: mycelia
stress: drought
treatment: none
|
| Treatment protocol |
Drought treatment was applied by stopping irrigation; samples were taken after 3 and 7 days, respectively;
|
| Growth protocol |
Agaricus bisporus hyphae on agar medium were placed on hay in gas-tight mesocosms and irrigated for three weeks with water or water containing 50 µM Riboflavin;
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Rneasy Microarray Tissue Kit (Qiagen GmbH, Hilden, Germany);
|
| Label |
Cy5
|
| Label protocol |
RNA was labelled using the Low Input Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, CA, USA);
|
| |
|
| Channel 2 |
| Source name |
mycelia, irrigated, day 7
|
| Organism |
Agaricus bisporus |
| Characteristics |
strain: var bisporus (H97)
tissue: mycelia
stress: irrigated
treatment: none
|
| Treatment protocol |
Drought treatment was applied by stopping irrigation; samples were taken after 3 and 7 days, respectively;
|
| Growth protocol |
Agaricus bisporus hyphae on agar medium were placed on hay in gas-tight mesocosms and irrigated for three weeks with water or water containing 50 µM Riboflavin;
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Rneasy Microarray Tissue Kit (Qiagen GmbH, Hilden, Germany);
|
| Label |
Cy3
|
| Label protocol |
RNA was labelled using the Low Input Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, CA, USA);
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing if the Microarrays was perfomed as described in the Low Input Quick Amp Labeling Kit (Agilent Technologies)
|
| Scan protocol |
Microarrays were scanned using a high-resolution scanner G2505C (Agilent Technologies) and Agilent's Feature Extraction software (v. 11.5.1.1) was used to quantify hybridization signals;
|
| Description |
Biological replicate 1 of 2, harvest day 7, dye-swap
|
| Data processing |
Data were processed using LIMMA: background correction (normexp), normalized within arrays (loess), normalized between arrays (Aquantile);
|
| |
|
| Submission date |
Sep 14, 2015 |
| Last update date |
Mar 14, 2018 |
| Contact name |
Alfons R Weig |
| E-mail(s) |
a.weig@uni-bayreuth.de
|
| Organization name |
University of Bayreuth
|
| Department |
Genomics and Bioinformatics
|
| Street address |
Universitaetsstrasse 30
|
| City |
Bayreuth |
| ZIP/Postal code |
95447 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20913 |
| Series (1) |
| GSE73010 |
Vitamin B2 and drought stress responses in Agaricus bisporus |
|
