|
| Status |
Public on Jun 12, 2017 |
| Title |
Rif8B vs Rif5B |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
ATCC 17978 derivative spontaneous mutant Rif8
|
| Organism |
Acinetobacter baumannii |
| Characteristics |
strain: ATCC 17978
genotype: spontaneous mutant Rif8
|
| Treatment protocol |
All strains were grown until the mid-exponential growth phase (OD600=0.6) was reached
|
| Growth protocol |
A. baumannii ATCC 17978 wild-type strain was grown in Luria Bertani (LB) medium and incubated at 37ºC with shaking at 180 rpm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy mini kit (Qiagen). DNA contaminants were removed from the RNA by digestion with DNase Turbo (Ambion).
|
| Label |
Cy5
|
| Label protocol |
Two-Color Microarray-Based Prokaryote Analysis Fair Play III Labeling v. 1.3 (Agilent)
|
| |
|
| Channel 2 |
| Source name |
ATCC 17978 derivative spontaneous mutant Rif5
|
| Organism |
Acinetobacter baumannii |
| Characteristics |
strain: ATCC 17978
genotype: spontaneous mutant Rif5
|
| Treatment protocol |
All strains were grown until the mid-exponential growth phase (OD600=0.6) was reached
|
| Growth protocol |
A. baumannii ATCC 17978 wild-type strain was grown in Luria Bertani (LB) medium and incubated at 37ºC with shaking at 180 rpm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy mini kit (Qiagen). DNA contaminants were removed from the RNA by digestion with DNase Turbo (Ambion).
|
| Label |
Cy3
|
| Label protocol |
Two-Color Microarray-Based Prokaryote Analysis Fair Play III Labeling v. 1.3 (Agilent)
|
| |
|
| |
| Hybridization protocol |
Microarrays were hybridized and washed according to manufacturer's protocol.
|
| Scan protocol |
Two-Color Microarray-Based Prokaryote Analysis 1.4
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1)
|
| |
|
| Submission date |
Sep 18, 2015 |
| Last update date |
Jun 13, 2017 |
| Contact name |
Jesús Aranda |
| E-mail(s) |
jesus.aranda@uab.cat
|
| Organization name |
Universitat Autònoma de Barcelona
|
| Department |
Departament de Genètica i Microbiologia
|
| Street address |
Facultat de Biociències, Plaza Cívica, s/n
|
| City |
Barcelona |
| ZIP/Postal code |
08193 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16729 |
| Series (1) |
| GSE73193 |
Characterization of two Acinetobacter baumannii rifampin-spontaneous mutants lacking twitching motility |
|
