|
| Status |
Public on Oct 31, 2016 |
| Title |
TM136 |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: primary ovarian tumor
age: 58
histology: 4
Stage: III
grading: undiff
debulking: mRD
ttr_time: 15
ttr_status: 1
os_time: 35
os_status: 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were mechanically disrupted and simultaneously homogenized in the presence of TRIzol Lysis reagent and using a dismembrator (Braun Biotech International, Melsungen, Germany). RNA was extracted using the miRNeasy Mini kit (Qiagen) according to manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
RNA from each sample was labeled according to the manufacturers recommendations (Illumina MicroRNA Expression Profiling Assay Guide).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were processed and normalised using quantile normalisation with BeadStudio software v3.8
|
| |
|
| Submission date |
Sep 29, 2015 |
| Last update date |
Oct 31, 2016 |
| Contact name |
Loris De Cecco |
| E-mail(s) |
loris.dececco@istitutotumori.mi.it
|
| Organization name |
IRCSS Istituto Nazionale Tumori
|
| Street address |
via Venezian 1
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8179 |
| Series (2) |
| GSE73582 |
MiROvaR, a microRNA-based predictor of early relapse for ovarian cancer patients [validation set; OC133] |
| GSE73584 |
MiROvaR, a microRNA-based predictor of early relapse for ovarian cancer patients |
|
