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| Status |
Public on Jul 17, 2007 |
| Title |
DLD Dicer -/- #2 transfected with Luc duplex versus control transfected DLD Dicer -/- #2, 24 hours |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mock transfected DLD Dicer -/- #2, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
Mock transfected DLD Dicer -/- #2, 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy
|
| Label |
Cy3
|
| Label protocol |
Customized Ambion MessageAmp II
|
| |
|
| Channel 2 |
| Source name |
DLD Dicer -/- #2 transfected with Luc duplex, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
DLD Dicer -/- #2 transfected with Luc duplex, 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy
|
| Label |
Cy5
|
| Label protocol |
Customized Ambion MessageAmp II
|
| |
|
| |
| Hybridization protocol |
Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7, PMID 11283592.
|
| Scan protocol |
Agilent LP2 laser scanner
|
| Description |
Transfection of duplexes into cell lines as described in He et al., Nature, 2007
|
| Data processing |
Error model as described in Hughes et al and in US patent #6,351,712
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| |
|
| Submission date |
May 21, 2007 |
| Last update date |
Jul 16, 2007 |
| Contact name |
Lee Lim |
| E-mail(s) |
lee_lim@merck.com
|
| Phone |
206-802-7323
|
| Organization name |
Rosetta Inpharmatics
|
| Street address |
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL4372 |
| Series (1) |
| GSE7864 |
A microRNA component of the p53 tumor suppressor network |
|
| Data table header descriptions |
| ID_REF |
Rosetta generated unique probe identifier |
| VALUE |
LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted] |
| LOG_INTENSITY |
Averaged log10 intensity of a fluor-reversed chip pair |
| P_VALUE |
P value that the probe target is differentially expressed in the two tissues |
| INTENSITY1 |
CHANNEL1 intensity; averaged raw data from a fluor-reversed pair |
| INTENSITY2 |
CHANNEL2 intensity; averaged raw data from a fluor-reversed pair |
| Data table |
| ID_REF |
VALUE |
LOG_INTENSITY |
P_VALUE |
INTENSITY1 |
INTENSITY2 |
| 10019475365 |
-0.023036463186144829 |
-0.8890000581741333 |
0.61682891845703125 |
0.13288500905036926 |
0.12599499523639679 |
| 10019481149 |
-0.0002359765931032598 |
-0.46544206142425537 |
0.99570703506469727 |
0.34681999683380127 |
0.34661000967025757 |
| 10019495284 |
-0.047817587852478027 |
1.0159196853637695 |
0.28860464692115784 |
10.96306037902832 |
9.8187847137451172 |
| 10019687586 |
-0.030092645436525345 |
-1.0863375663757324 |
0.56851816177368164 |
0.08487999439239502 |
0.079350002110004425 |
| 10019713746 |
0.027888141572475433 |
-1.0942119359970093 |
0.62579184770584106 |
0.078055001795291901 |
0.083080001175403595 |
| 10019799479 |
-0.0059284917078912258 |
-0.957358717918396 |
0.91183251142501831 |
0.1118600070476532 |
0.11071000248193741 |
| 10019809115 |
0.010757213458418846 |
-0.38310331106185913 |
0.85860025882720947 |
0.40986001491546631 |
0.42367500066757202 |
| 10019874890 |
-0.018201049417257309 |
-0.99781614542007446 |
0.71507024765014648 |
0.10378500074148178 |
0.099209994077682495 |
| 10019903058 |
0.041762389242649078 |
-1.4411437511444092 |
0.65262120962142944 |
0.035980001091957092 |
0.039524998515844345 |
| 10019909307 |
0.038060486316680908 |
-1.4262259006500244 |
0.57308381795883179 |
0.035855002701282501 |
0.039269998669624329 |
| 10019911222 |
-0.0092917103320360184 |
1.0232632160186768 |
0.8723340630531311 |
10.664275169372559 |
10.455934524536133 |
| 10019924807 |
-0.065612785518169403 |
-1.8104411363601685 |
0.53347063064575195 |
0.016854999586939812 |
0.014259999617934227 |
| 10019927856 |
0.013240000233054161 |
0.70816558599472046 |
0.91420996189117432 |
5.0297598838806152 |
5.185420036315918 |
| 10019932383 |
-0.091713115572929382 |
-0.37783995270729065 |
0.13438889384269714 |
0.46595001220703125 |
0.37738001346588135 |
| 10019948931 |
-0.065313845872879028 |
-1.5198853015899658 |
0.36840590834617615 |
0.032674998044967651 |
0.027989998459815979 |
| 10019975533 |
-0.048326831310987473 |
-1.5596821308135986 |
0.53914058208465576 |
0.031039997935295105 |
0.028094999492168427 |
| 10019977224 |
-0.01840023510158062 |
0.82505965232849121 |
0.7430497407913208 |
6.8309402465820313 |
6.5463900566101074 |
| 10019977227 |
-0.065334126353263855 |
-0.62329649925231934 |
0.24902401864528656 |
0.25754499435424805 |
0.22289499640464783 |
| 10019987588 |
0.025476625189185143 |
-1.0953290462493896 |
0.60247904062271118 |
0.077979996800422668 |
0.082674995064735413 |
| 10020008603 |
-0.0019457434536889195 |
-1.1082749366760254 |
0.97113168239593506 |
0.078199997544288635 |
0.07807999849319458 |
Total number of rows: 39302
Table truncated, full table size 4372 Kbytes.
| Supplementary data files not provided |
| Processed data included within Sample table |
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