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| Status |
Public on Jul 17, 2007 |
| Title |
DLD Dicer -/- #2 transfected with miR_34c duplex versus control transfected DLD Dicer -/- #2, 24 hours |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mock transfected DLD Dicer -/- #2, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
Mock transfected DLD Dicer -/- #2, 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy
|
| Label |
Cy3
|
| Label protocol |
Customized Ambion MessageAmp II
|
| |
|
| Channel 2 |
| Source name |
DLD Dicer -/- #2 transfected with miR_34c duplex, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
DLD Dicer -/- #2 transfected with miR_34c duplex, 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy
|
| Label |
Cy5
|
| Label protocol |
Customized Ambion MessageAmp II
|
| |
|
| |
| Hybridization protocol |
Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7, PMID 11283592.
|
| Scan protocol |
Agilent LP2 laser scanner
|
| Description |
Transfection of duplexes into cell lines as described in He et al., Nature, 2007
|
| Data processing |
Error model as described in Hughes et al and in US patent #6,351,712
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| |
|
| Submission date |
May 21, 2007 |
| Last update date |
Jul 16, 2007 |
| Contact name |
Lee Lim |
| E-mail(s) |
lee_lim@merck.com
|
| Phone |
206-802-7323
|
| Organization name |
Rosetta Inpharmatics
|
| Street address |
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL4372 |
| Series (1) |
| GSE7864 |
A microRNA component of the p53 tumor suppressor network |
|
| Data table header descriptions |
| ID_REF |
Rosetta generated unique probe identifier |
| VALUE |
LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted] |
| LOG_INTENSITY |
Averaged log10 intensity of a fluor-reversed chip pair |
| P_VALUE |
P value that the probe target is differentially expressed in the two tissues |
| INTENSITY1 |
CHANNEL1 intensity; averaged raw data from a fluor-reversed pair |
| INTENSITY2 |
CHANNEL2 intensity; averaged raw data from a fluor-reversed pair |
| Data table |
| ID_REF |
VALUE |
LOG_INTENSITY |
P_VALUE |
INTENSITY1 |
INTENSITY2 |
| 10019475365 |
-0.01888618990778923 |
-0.46857628226280212 |
0.72420603036880493 |
0.3474850058555603 |
0.33276998996734619 |
| 10019481149 |
0.00054500019177794456 |
-0.027639053761959076 |
0.99104428291320801 |
0.93775999546051025 |
0.93927997350692749 |
| 10019495284 |
-0.4113675057888031 |
0.79062080383300781 |
6.856936685117887e-010 |
9.9427204132080078 |
3.840789794921875 |
| 10019687586 |
0.10237811505794525 |
-1.0582593679428101 |
0.044109988957643509 |
0.078299999237060547 |
0.099240005016326904 |
| 10019713746 |
-0.017692022025585175 |
-0.65464353561401367 |
0.71209526062011719 |
0.22617998719215393 |
0.21706999838352203 |
| 10019799479 |
0.020674072206020355 |
-0.6187477707862854 |
0.64386653900146484 |
0.23511999845504761 |
0.24663498997688293 |
| 10019809115 |
-0.00016000121831893921 |
0.28855323791503906 |
0.99818938970565796 |
2.001694917678833 |
1.9568901062011719 |
| 10019874890 |
-0.012057296931743622 |
-0.50179076194763184 |
0.78997713327407837 |
0.31933498382568359 |
0.31060999631881714 |
| 10019903058 |
0.094976693391799927 |
-1.5473711490631104 |
0.47639763355255127 |
0.026429999619722366 |
0.031275000423192978 |
| 10019909307 |
-0.0010005156509578228 |
-0.87441420555114746 |
0.98454290628433228 |
0.13424499332904816 |
0.13446000218391418 |
| 10019911222 |
0.018501052632927895 |
0.97386348247528076 |
0.70507752895355225 |
9.2273654937744141 |
9.6433506011962891 |
| 10019924807 |
-0.0022856113500893116 |
-1.4711477756500244 |
0.97508132457733154 |
0.03405500203371048 |
0.033590000122785568 |
| 10019927856 |
-0.011135749518871307 |
0.61485546827316284 |
0.81853765249252319 |
4.2388801574707031 |
4.115684986114502 |
| 10019932383 |
-0.06358395516872406 |
-0.46483272314071655 |
0.29270541667938232 |
0.36989998817443848 |
0.31862500309944153 |
| 10019948931 |
0.021203510463237762 |
-1.0440037250518799 |
0.73792296648025513 |
0.089634999632835388 |
0.092979997396469116 |
| 10019975533 |
-0.030351307243108749 |
-1.3116071224212646 |
0.67274290323257446 |
0.050884999334812164 |
0.047380000352859497 |
| 10019977224 |
0.10772856324911118 |
0.84383487701416016 |
0.067725725471973419 |
6.173065185546875 |
7.9053049087524414 |
| 10019977227 |
-0.033717203885316849 |
-0.60658103227615356 |
0.69340705871582031 |
0.26090499758720398 |
0.23773999512195587 |
| 10019987588 |
-0.00898006372153759 |
-0.65806430578231812 |
0.87069958448410034 |
0.22246000170707703 |
0.21894499659538269 |
| 10020008603 |
-0.0064632678404450417 |
-0.61360704898834229 |
0.88509118556976318 |
0.24527500569820404 |
0.24165999889373779 |
Total number of rows: 39302
Table truncated, full table size 4370 Kbytes.
| Supplementary data files not provided |
| Processed data included within Sample table |
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