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| Status |
Public on Jul 17, 2007 |
| Title |
A549 p53-1026 transfected with miR34b duplex versus control transfected A549 p53-1026, 24 hours |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mock transfected A549 p53-1026, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
Mock transfected A549 p53-1026, 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy
|
| Label |
Cy3
|
| Label protocol |
Customized Ambion MessageAmp II
|
| |
|
| Channel 2 |
| Source name |
A549 p53-1026 transfected with miR34b duplex, 24 hours
|
| Organism |
Homo sapiens |
| Characteristics |
A549 p53-1026 transfected with miR34b duplex, 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNAeasy
|
| Label |
Cy5
|
| Label protocol |
Customized Ambion MessageAmp II
|
| |
|
| |
| Hybridization protocol |
Hybridization to Agilent microarrays as described in Hughes et al, Nature Biotechnology (2001), 19(4):342-7, PMID 11283592.
|
| Scan protocol |
Agilent LP2 laser scanner
|
| Description |
Transfection of duplexes into cell lines as described in He et al., Nature, 2007
|
| Data processing |
Error model as described in Hughes et al and in US patent #6,351,712
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| |
|
| Submission date |
May 21, 2007 |
| Last update date |
Jul 16, 2007 |
| Contact name |
Lee Lim |
| E-mail(s) |
lee_lim@merck.com
|
| Phone |
206-802-7323
|
| Organization name |
Rosetta Inpharmatics
|
| Street address |
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL4372 |
| Series (1) |
| GSE7864 |
A microRNA component of the p53 tumor suppressor network |
|
| Data table header descriptions |
| ID_REF |
Rosetta generated unique probe identifier |
| VALUE |
LOG10 RATIO: Averaged log10 ratio of a fluor-reversed chip pair [log10(CHANNEL2/CHANNEL1), error weighted] |
| LOG_INTENSITY |
Averaged log10 intensity of a fluor-reversed chip pair |
| P_VALUE |
P value that the probe target is differentially expressed in the two tissues |
| INTENSITY1 |
CHANNEL1 intensity; averaged raw data from a fluor-reversed pair |
| INTENSITY2 |
CHANNEL2 intensity; averaged raw data from a fluor-reversed pair |
| Data table |
| ID_REF |
VALUE |
LOG_INTENSITY |
P_VALUE |
INTENSITY1 |
INTENSITY2 |
| 10019475365 |
-0.015132497064769268 |
-0.49483707547187805 |
0.7377057671546936 |
0.32892501354217529 |
0.31798499822616577 |
| 10019481149 |
-0.031495042145252228 |
-0.37999048829078674 |
0.61400055885314941 |
0.43261000514030457 |
0.40475499629974365 |
| 10019495284 |
-0.24060443043708801 |
0.31877350807189941 |
0.0017086493317037821 |
2.7644200325012207 |
1.5767500400543213 |
| 10019687586 |
0.0050853267312049866 |
-1.0620520114898682 |
0.92352509498596191 |
0.087115004658699036 |
0.088534995913505554 |
| 10019713746 |
0.025079147890210152 |
-0.38881552219390869 |
0.63447290658950806 |
0.41010499000549316 |
0.43031001091003418 |
| 10019799479 |
-0.0015635831514373422 |
-0.67367994785308838 |
0.97387617826461792 |
0.21270999312400818 |
0.21218499541282654 |
| 10019809115 |
0.006968500092625618 |
0.057989563792943954 |
0.88657152652740479 |
1.1588000059127808 |
1.1718399524688721 |
| 10019874890 |
-0.016858501359820366 |
-0.60842418670654297 |
0.75571542978286743 |
0.2515299916267395 |
0.24162000417709351 |
| 10019903058 |
0.079435467720031738 |
-1.146597146987915 |
0.50918090343475342 |
0.065080001950263977 |
0.08362000435590744 |
| 10019909307 |
-0.0036876299418509007 |
-1.083482027053833 |
0.94044786691665649 |
0.08582499623298645 |
0.08487999439239502 |
| 10019911222 |
-0.04968000203371048 |
0.51981759071350098 |
0.26239770650863647 |
3.5054700374603271 |
3.1263599395751953 |
| 10019924807 |
0.0073469639755785465 |
-1.3833630084991455 |
0.89079552888870239 |
0.041110001504421234 |
0.041825000196695328 |
| 10019927856 |
-0.11103056371212006 |
0.60387074947357178 |
0.17384172976016998 |
4.5779800415039062 |
3.5830550193786621 |
| 10019932383 |
0.023719735443592072 |
-0.12709373235702515 |
0.71012639999389648 |
0.73813998699188232 |
0.77024000883102417 |
| 10019948931 |
0.015482465736567974 |
-1.0475220680236816 |
0.84273171424865723 |
0.093805000185966492 |
0.093594998121261597 |
| 10019975533 |
0.036569211632013321 |
-1.1513862609863281 |
0.45100709795951843 |
0.06821499764919281 |
0.074149996042251587 |
| 10019977224 |
0.0068979999050498009 |
0.4492301344871521 |
0.95520424842834473 |
2.791140079498291 |
2.8358199596405029 |
| 10019977227 |
-0.02632611058652401 |
-0.36648666858673096 |
0.77448880672454834 |
0.44538000226020813 |
0.42048001289367676 |
| 10019987588 |
-0.0011272846022620797 |
-0.87973892688751221 |
0.98065584897994995 |
0.1358799934387207 |
0.13583000004291534 |
| 10020008603 |
-0.0044790599495172501 |
-0.57162636518478394 |
0.94086289405822754 |
0.27973499894142151 |
0.27201500535011292 |
Total number of rows: 39302
Table truncated, full table size 4361 Kbytes.
| Supplementary data files not provided |
| Processed data included within Sample table |
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