|
| Status |
Public on Jan 01, 2016 |
| Title |
early ovary-Orb_IP-rep5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Orb IP , ovaries from newly eclosed females
|
| Organism |
Drosophila melanogaster |
| Characteristics |
antibody: Orb (6H4 (DSHB) )
tissue: ovary
developmental stage: newly eclosed
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
not provided
|
| Label |
Cy3
|
| Label protocol |
RNA were labelled with Cy3 or Cy5 using the Ambion kit Amino Allyl Message Amp II.
|
| |
|
| Channel 2 |
| Source name |
mock IP, ovaries from newly eclosed females
|
| Organism |
Drosophila melanogaster |
| Characteristics |
antibody: commercial mouse IgGs
tissue: ovary
developmental stage: newly eclosed
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
not provided
|
| Label |
Cy5
|
| Label protocol |
RNA were labelled with Cy3 or Cy5 using the Ambion kit Amino Allyl Message Amp II.
|
| |
|
| |
| Hybridization protocol |
Cy3 and Cy5 labelled aRNA samples were mixed in equal proportions and hybridized at 37°C for 12 hours with the Automated Slide Processor (ASP) on INDAC oligonucleotide microarrays.
|
| Scan protocol |
Microarrays were immediately scanned at 10 µm resolution in both Cy3 and Cy5 channels with a GenePix 4200AL scanner (Molecular Devices) with a variable PMT voltage to obtain maximal signal intensities. ArrayVision software was used for feature extraction.
|
| Description |
Orb RIP-chip
|
| Data processing |
We performed Significant Analysis of Microarrays (SAM) with False Discovery Rate (FDR) 0.01 to identify mRNAs that were enriched at least 1.5 fold in Orb RIP compared to mock RIP.
|
| |
|
| Submission date |
Oct 29, 2015 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Simonelig Martine |
| E-mail(s) |
martine.simonelig@igh.cnrs.fr
|
| Organization name |
Institut de Genetique Humaine - CNRS
|
| Department |
Genetics and Development
|
| Lab |
mRNA Regulation and Development
|
| Street address |
141, rue de la Cardonille
|
| City |
MONTPELLIER |
| ZIP/Postal code |
34396 |
| Country |
France |
| |
|
| Platform ID |
GPL5112 |
| Series (2) |
| GSE74474 |
Translational control of autophagy by Orb in the Drosophila germline [early] |
| GSE74476 |
Translational control of autophagy by Orb in the Drosophila germline |
|
