|
| Status |
Public on Apr 11, 2019 |
| Title |
H9 embryonic stem cells, Ncad negative SSEA3 positive sorted population, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
H9 embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
sorted cells: Ncad negative SSEA3 positive
|
| Growth protocol |
MEF conditioned medium + 8ng/ml bFGF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA using total RNA purification kit (Norgen Biotek, Canada) was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
In accordance with manufacturer's protocols
|
| |
|
| Hybridization protocol |
In accordance with manufacturer's protocols
|
| Scan protocol |
GeneChips were scanned using Affymetrix® Scanner 3000 7G.
|
| Data processing |
Data was normalized using Robust Multichip Averaging (RMA) algorithm using Partek Genomics Suite 6.6 software.
|
| |
|
| Submission date |
Nov 13, 2015 |
| Last update date |
Apr 12, 2019 |
| Contact name |
Mickie Bhatia |
| E-mail(s) |
mbhatia@mcmaster.ca
|
| Organization name |
McMaster University
|
| Department |
Stem Cell and Cancer Research Institute (SCC-RI)
|
| Street address |
1200 Main Street West
|
| City |
Hamilton |
| State/province |
Ontario |
| ZIP/Postal code |
L8N 3Z5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE74990 |
Human pluripotency is initiated and preserved by a unique subset of founder cells [microarray] |
|
