|
Status |
Public on Jun 30, 2008 |
Title |
TMK4_WT_II |
Sample type |
genomic |
|
|
Source name |
14 day old seedlings
|
Organism |
Arabidopsis thaliana |
Characteristics |
Enriched for DNA associated with trimethylation of H3 K4 Aerial parts of 14 day old seedlings
|
Treatment protocol |
Untreated
|
Growth protocol |
Plants were grown under 16h light on soil
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Chromatin immunoprecipitation using anti-trimethylation of H3 K4 antibody (Upstate)
|
Label |
biotin
|
Label protocol |
Biotinylated DNA was prepared according to the Affymetrix® Chromatin Immunoprecipitation Assay Protocol (P/N 702238 Rev. 2, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, DNA was hybridized for 16 hr on GeneChip Arabidopsis Tiling 1.0R Array. GeneChips were washed and stained in the Affymetrix Fluidics Station according to the standard Affymetrix protocol.
|
Scan protocol |
GeneChips were scanned using The GeneChip Scanner 3000 7G
|
Description |
Enriched for DNA associated with trimethylation of H3 K4
|
Data processing |
Quantile Normalization using TileMap package
|
|
|
Submission date |
May 25, 2007 |
Last update date |
Dec 21, 2007 |
Contact name |
Sookyung Oh |
E-mail(s) |
ohsookyu@msu.edu
|
Phone |
517-355-5191-369
|
Organization name |
Michigan State University
|
Department |
Horticulture
|
Lab |
Steve van Nocker
|
Street address |
|
City |
East Lansing |
State/province |
MI |
ZIP/Postal code |
48824 |
Country |
USA |
|
|
Platform ID |
GPL1979 |
Series (1) |
GSE7907 |
Histone H3 modifications ChIP on chip |
|