|
| Status |
Public on Feb 17, 2017 |
| Title |
Thy1+LSK sorted bone marrow cells from NOL3+/+ mice rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Thy1+LSK sorted bone marrow cells from NOL3+/+ mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Thy1+LSK sorted bone marrow cells from NOL3+/+ mice
genotype: NOL3+/+
background strain: C57BL/6
|
| Treatment protocol |
No treatment
|
| Growth protocol |
uncultured primary cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNA was extracted using Qiagen's RNAeasy Micro kit according to the manufacturer's instructions; After checking the quality of the RNA with an Agilent 2100 Bioanalyzer, total RNA was amplified utilizing Single Primer Isothermal Amplification (SPIA Nugen Ovation pico WTA) system according the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the GeneChip WT terminal labeling kit (Affymetrix) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Labeld cRNA of each individual samples was hybridized to Affymetrix Mouse Gene 2.0 ST microarray
|
| Scan protocol |
Scanned by GeneChip Scanner 3000 7G System (Affymetrix) according to standard protocols.
|
| Description |
Gene expression data
|
| Data processing |
Affymetrix Expression Console Software and Transcriptome Analysis Console
|
| |
|
| Submission date |
Dec 17, 2015 |
| Last update date |
Feb 19, 2017 |
| Contact name |
Robert Stanley |
| E-mail(s) |
robert.f.stanley@med.einstein.yu.edu
|
| Phone |
7184303788
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Cell Biology
|
| Lab |
Ulrich Steidl
|
| Street address |
1300 Morris Park Avenue, Chanin Building Rm 601
|
| City |
Bronx |
| State/province |
New York |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE76121 |
Gene expression analysis of Thy1+LSK bone marrow cells from Nol3+/+ and Nol3-/- MPN mice |
|
