|
Status |
Public on Jan 04, 2016 |
Title |
cancer [2526] |
Sample type |
RNA |
|
|
Source name |
breast_TNBC
|
Organism |
Homo sapiens |
Characteristics |
gender: female age (yrs): 41 menopause status (0=pre, 1=post): 0 size (cm): 3 tumor grade: 2 positive lymph nodes: 14 ki67(%): 25 tissue type: triple negative breast cancer (TNBC)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from cells at indicated time point with Trizol
|
Label |
biotin
|
Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA by using Ambion® WT Expression Kit. WT Expression Kit
|
|
|
Hybridization protocol |
Following labeling, 5.5 ug of cDNA were hybridized for 16 hr at 45℃ on GeneChip Human Transcriptome Array 2.0 in Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
Scan protocol |
GeneChips were scaned by using Affymetrix® GeneChip Command Console (AGCC) which installed in GeneChip® Scanner 3000 7G
|
Description |
2526_(HTA-2_0).cel
|
Data processing |
The data were analyzed with Robust Multichip Analysis (RMA) algorithm using Affymetrix default analysis settings and global scaling as normalization method (ExpressionConsole 1.3.1) probe group file: HTA-2_0.r1.pgf meta-probeset file: HTA-2_0.r1.Psrs.mps
|
|
|
Submission date |
Dec 22, 2015 |
Last update date |
Jan 05, 2016 |
Contact name |
Yi-Zhou Jiang |
E-mail(s) |
yizhoujiang@fudan.edu.cn
|
Phone |
+8615921910483
|
Organization name |
Fudan University Shanghai Cancer Center
|
Street address |
No. 270 Dong-An Road
|
City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
|
|
Platform ID |
GPL17586 |
Series (1) |
GSE76250 |
Comprehensive transcriptome profiling reveals multigene signatures in triple negative breast cancer |
|