|
| Status |
Public on Jan 04, 2016 |
| Title |
cancer [4700+] |
| Sample type |
RNA |
| |
|
| Source name |
breast_TNBC
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age (yrs): 57
menopause status (0=pre, 1=post): 1
size (cm): 1.8
tumor grade: 3
positive lymph nodes: 0
ki67(%): 70
tissue type: triple negative breast cancer (TNBC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells at indicated time point with Trizol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA by using Ambion® WT Expression Kit. WT Expression Kit
|
| |
|
| Hybridization protocol |
Following labeling, 5.5 ug of cDNA were hybridized for 16 hr at 45℃ on GeneChip Human Transcriptome Array 2.0 in Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scaned by using Affymetrix® GeneChip Command Console (AGCC) which installed in GeneChip® Scanner 3000 7G
|
| Description |
4700+_(HTA-2_0).cel
|
| Data processing |
The data were analyzed with Robust Multichip Analysis (RMA) algorithm using Affymetrix default analysis settings and global scaling as normalization method (ExpressionConsole 1.3.1)
probe group file: HTA-2_0.r1.pgf
meta-probeset file: HTA-2_0.r1.Psrs.mps
|
| |
|
| Submission date |
Dec 22, 2015 |
| Last update date |
Jan 05, 2016 |
| Contact name |
Yi-Zhou Jiang |
| E-mail(s) |
yizhoujiang@fudan.edu.cn
|
| Phone |
+8615921910483
|
| Organization name |
Fudan University Shanghai Cancer Center
|
| Street address |
No. 270 Dong-An Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE76250 |
Comprehensive transcriptome profiling reveals multigene signatures in triple negative breast cancer |
|
