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Sample GSM1977806 Query DataSets for GSM1977806
Status Public on Jan 04, 2016
Title cancer [4791+]
Sample type RNA
 
Source name breast_TNBC
Organism Homo sapiens
Characteristics gender: female
age (yrs): 50
menopause status (0=pre, 1=post): 0
size (cm): 2
tumor grade: 0
positive lymph nodes: 0
ki67(%): 25
tissue type: triple negative breast cancer (TNBC)
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cells at indicated time point with Trizol
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA by using Ambion® WT Expression Kit. WT Expression Kit
 
Hybridization protocol Following labeling, 5.5 ug of cDNA were hybridized for 16 hr at 45℃ on GeneChip Human Transcriptome Array 2.0 in Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scaned by using Affymetrix® GeneChip Command Console (AGCC) which installed in GeneChip® Scanner 3000 7G
Description 4791+_(HTA-2_0).cel
Data processing The data were analyzed with Robust Multichip Analysis (RMA) algorithm using Affymetrix default analysis settings and global scaling as normalization method (ExpressionConsole 1.3.1)
probe group file: HTA-2_0.r1.pgf
meta-probeset file: HTA-2_0.r1.Psrs.mps
 
Submission date Dec 22, 2015
Last update date Jan 05, 2016
Contact name Yi-Zhou Jiang
E-mail(s) yizhoujiang@fudan.edu.cn
Phone +8615921910483
Organization name Fudan University Shanghai Cancer Center
Street address No. 270 Dong-An Road
City Shanghai
State/province Shanghai
ZIP/Postal code 200032
Country China
 
Platform ID GPL17586
Series (1)
GSE76250 Comprehensive transcriptome profiling reveals multigene signatures in triple negative breast cancer

Data table header descriptions
ID_REF
VALUE normalized

Data table
ID_REF VALUE
TC01000001.hg.1 4.251326
TC01000002.hg.1 3.595412
TC01000003.hg.1 1.647896
TC01000004.hg.1 1.846134
TC01000005.hg.1 5.991062
TC01000006.hg.1 9.619781
TC01000007.hg.1 9.241955
TC01000008.hg.1 5.362765
TC01000009.hg.1 1.41547
TC01000010.hg.1 3.148393
TC01000011.hg.1 5.225954
TC01000012.hg.1 3.768028
TC01000013.hg.1 6.278003
TC01000014.hg.1 2.102454
TC01000015.hg.1 4.498136
TC01000016.hg.1 3.933264
TC01000017.hg.1 4.462245
TC01000018.hg.1 5.65744
TC01000019.hg.1 5.399866
TC01000020.hg.1 5.770485

Total number of rows: 70523

Table truncated, full table size 1726 Kbytes.




Supplementary file Size Download File type/resource
GSM1977806_4791+_HTA-2_0_.cel.gz 22.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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