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| Status |
Public on Aug 01, 2017 |
| Title |
RNA of Rapeseed Pollen |
| Sample type |
SRA |
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| Source name |
Rapeseed Pollen
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| Organism |
Apis mellifera |
| Characteristics |
product/source: Pollen
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 10g of Royal Jelly/Honey/Bee Bread/Pollen using the Trizol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer IIx |
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| Data processing |
Illumina Casava1.6 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then aligned to pre-miRNA sequence in miRBase_v21
Sequenced reads with no mismatches and no more than 2 shifts were identified as one miRNA and formed the final miRNA expression profiles.
Genome_build: miRBase_v21
Supplementary_files_format_and_content: tab-delimited text files include microRNA names, sequences and counts in each sample
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| Submission date |
Dec 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Zhen Zhou |
| E-mail(s) |
darknesszhou@gmail.com
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| Organization name |
Nanjing University
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| Department |
School of Lifesciences
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| Street address |
22th,Hankou Road
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210093 |
| Country |
China |
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| Platform ID |
GPL13725 |
| Series (1) |
| GSE76286 |
Plant microRNAs in larval food regulate honeybee caste development |
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| Relations |
| BioSample |
SAMN04364428 |
| SRA |
SRX1499206 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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