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| Status |
Public on Aug 01, 2017 |
| Title |
Plant microRNAs in larval food regulate honeybee caste development |
| Organisms |
Camellia japonica; Apis mellifera |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The major environmental determinants of honeybee caste development come from larval nutrients: royal jelly stimulates the differentiation of larvae into queens, whereas beebread leads to worker bee fate. However, these determinants are not fully characterized. Here we report that plant RNAs, particularly miRNAs, which are more enriched in beebread than in royal jelly, delay development and decrease body and ovary size in honeybees, thereby preventing larval differentiation into queens and inducing development into worker bees. Mechanistic studies reveal that amTOR, a stimulatory gene in caste differentiation, is the direct target of miR162a. Interestingly, the same effect also exists in non-social Drosophila. When such plant RNAs and miRNAs are fed to Drosophila larvae, they cause extended developmental times and reductions in body weight and length, ovary size and fecundity. This study identifies an uncharacterized function of plant miRNAs that fine-tunes honeybee caste development, offering hints for understanding cross-kingdom interaction and coevolution.
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| Overall design |
Fresh samples were collected and stored at -80℃. Total RNA of Royal Jelly, Honey, Beebread and Pollen were extracted and solexa sequencing was performed.
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| Contributor(s) |
Zhu K, Fu Z, Zhou Z, Chen X |
| Citation(s) |
28859085 |
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| Submission date |
Dec 23, 2015 |
| Last update date |
Jul 10, 2019 |
| Contact name |
Zhen Zhou |
| E-mail(s) |
darknesszhou@gmail.com
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| Organization name |
Nanjing University
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| Department |
School of Lifesciences
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| Street address |
22th,Hankou Road
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210093 |
| Country |
China |
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| Platforms (2) |
| GPL13725 |
Illumina Genome Analyzer IIx (Apis mellifera) |
| GPL23850 |
Illumina HiSeq 4000 (Camellia japonica) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA306823 |
| SRA |
SRP067734 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE76286_processed_data_Camelia.txt.gz |
4.2 Kb |
(ftp)(http) |
TXT |
| GSE76286_processed_file_Rapeseed.txt.gz |
2.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data are available in SRA |
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