|
| Status |
Public on Dec 30, 2015 |
| Title |
mice heart ctl_rep-2 |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6 mice' heart was performed sham operation for 4 weeks
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: heart
|
| Treatment protocol |
The heart tissues were collected and stored in liquid nitrogen.
|
| Growth protocol |
The heart tissues were collected and stored in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacture's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinglated ss-ccDNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (Genechip WT PLUS reagent manual 2013)
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5μg of ss-ccDNA were hybridized for 16hr at 45C GeneChip transcriptome assays 1.0. Genechips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Genechip Scanner 3000 7G.
|
| Description |
Gene expression data from the heart performed the sham operation
|
| Data processing |
The data were analyzed with Affymetrix Expression Console Software using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Dec 29, 2015 |
| Last update date |
Dec 30, 2015 |
| Contact name |
Xuefeng Qu |
| E-mail(s) |
914316764@qq.com
|
| Phone |
+86 18045183552
|
| Organization name |
Harbin Medical University
|
| Department |
Department of Pharmacology
|
| Street address |
157 Baojian Road
|
| City |
Harbin |
| State/province |
Heilongjiang |
| ZIP/Postal code |
150081 |
| Country |
China |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE76387 |
Expression of lncRNAs in mouse heart 4 weeks after myocardial infarction |
|
