|
| Status |
Public on Jun 22, 2007 |
| Title |
high salt, 7 minutes A |
| Sample type |
RNA |
| |
|
| Source name |
photosynthetic conditions (10W/m2 white light illumination, no oxygen); high salt stress (1.5% NaCl) during 7 min
|
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Characteristics |
strain: 2.4.1
|
| Growth protocol |
Cells are grown at anoxic photosynthetic (10 W/m2 illumination) conditions; salt stress was induced by the addition of NaCl to the exponentially grown (A600 = 0.4 ± 0.03) cultures to the final concentration of 1.5 %; Cultures were collected at 7 min after addition of NaCl
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolated with bead beater + RNeasy kits
|
| Label |
biotin
|
| Label protocol |
according to Affymetrix protocol
|
| |
|
| Hybridization protocol |
according to Affymetrix protocol
|
| Scan protocol |
according to Affymetrix protocol
|
| Description |
Rhodobacter sphaeroides 2.4.1, Laramie
|
| Data processing |
RMA (context-dependent) processing for corresponding experimental series was used for data analysis and mentioned in the papers; MAS 5.0 values are depositied here for consistensy with other submissions
|
| |
|
| Submission date |
Jun 11, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Mark Gomelsky |
| E-mail(s) |
gomelsky@uwyo.edu
|
| Phone |
307-766-3522
|
| Organization name |
University of Wyoming
|
| Department |
Molecular Biology
|
| Lab |
Gomelsky
|
| Street address |
|
| City |
Laramie |
| State/province |
WY |
| ZIP/Postal code |
82071 |
| Country |
USA |
| |
|
| Platform ID |
GPL162 |
| Series (1) |
| GSE8082 |
Rhodobacter sphaeroides salt stress response |
|
