|
Status |
Public on Jun 22, 2007 |
Title |
high salt, 7 minutes B |
Sample type |
RNA |
|
|
Source name |
photosynthetic conditions (10W/m2 white light illumination, no oxygen); high salt stress (1.5% NaCl) during 7 min
|
Organism |
Cereibacter sphaeroides 2.4.1 |
Characteristics |
strain: 2.4.1
|
Growth protocol |
Cells are grown at anoxic photosynthetic (10 W/m2 illumination) conditions; salt stress was induced by the addition of NaCl to the exponentially grown (A600 = 0.4 ± 0.03) cultures to the final concentration of 1.5 %; Cultures were collected at 7 min after addition of NaCl
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA isolated with bead beater + RNeasy kits
|
Label |
biotin
|
Label protocol |
according to Affymetrix protocol
|
|
|
Hybridization protocol |
according to Affymetrix protocol
|
Scan protocol |
according to Affymetrix protocol
|
Description |
Rhodobacter sphaeroides 2.4.1, Laramie
|
Data processing |
RMA (context-dependent) processing for corresponding experimental series was used for data analysis and mentioned in the papers; MAS 5.0 values are depositied here for consistensy with other submissions
|
|
|
Submission date |
Jun 11, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Mark Gomelsky |
E-mail(s) |
gomelsky@uwyo.edu
|
Phone |
307-766-3522
|
Organization name |
University of Wyoming
|
Department |
Molecular Biology
|
Lab |
Gomelsky
|
Street address |
|
City |
Laramie |
State/province |
WY |
ZIP/Postal code |
82071 |
Country |
USA |
|
|
Platform ID |
GPL162 |
Series (1) |
GSE8082 |
Rhodobacter sphaeroides salt stress response |
|