|
| Status |
Public on Nov 14, 2007 |
| Title |
Placenta Replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Placenta total RNA
|
| Organism |
Homo sapiens |
| Characteristics |
Pooled Human placenta total RNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Purchased total RNA was purified further with an RNeasy Mini Kit (QIAGEN) and RNase-free DNase Set (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
Amino Allyl aRNA was synthesized by Amino Allyl MessageAmp™ aRNA Amplification Kit (Ambion). CyeDye coupling and fragmentation were performed using the protocol supplied by Hitachi Software Engineering Co., Ltd. (DNA Chip Research Inc. / AceGene website)
|
| |
|
| Channel 2 |
| Source name |
Universal Tissue Mix
|
| Organism |
Homo sapiens |
| Characteristics |
Mixture of RNA with the equal amount from each tissues aRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Purchased total RNA was purified further with an RNeasy Mini Kit (QIAGEN) and RNase-free DNase Set (QIAGEN).
|
| Label |
Cy5
|
| Label protocol |
Amino Allyl aRNA was synthesized by Amino Allyl MessageAmp™ aRNA Amplification Kit (Ambion). CyeDye coupling and fragmentation were performed using the protocol supplied by Hitachi Software Engineering Co., Ltd. (DNA Chip Research Inc. / AceGene website)
|
| |
|
| |
| Hybridization protocol |
Hybridized for 20 h at 50 °C. Hybridization buffer and washing protocol was followed by the protocol supplied by Hitachi Software Engineering Co., Ltd. (DNA Chip Research Inc. / AceGene website)
|
| Scan protocol |
GenePix4100A (Axon) was used for scanning.
Images were quantified using GenePixPro Software (version 4.1, Axon).
|
| Description |
Dye-swap replicate 1 of 2. Comparizon with universal tissue mix to placenta.
|
| Data processing |
Log2-ratios of the median subtracted background intensity levels were analyzed. Data from each microarray were normalized by LOWESS normalization using R software.
|
| |
|
| Submission date |
Jun 14, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Fuyuki Miya |
| E-mail(s) |
fmiya@keio.jp
|
| Phone |
+81-3-5363-3890
|
| Organization name |
Keio University
|
| Department |
School of Medicine
|
| Lab |
Center for Medical Genetics
|
| Street address |
35 Shinanomachi, Shinjuku-ku
|
| City |
Tokyo |
| State/province |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1291 |
| Series (1) |
| GSE8124 |
Gene expression profiles of 25 normal human tissues |
|
