|
| Status |
Public on Jul 20, 2016 |
| Title |
Host-P3_13_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Pseudomonas aeruginosa PAK
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
subsp.: Host PAK
strain: PAK
time point: 13 min post infection
|
| Treatment protocol |
Synchronous infection with bacteriophage PAK_P3 for 0min, 3,5min or 13min (MOI = 50) and halted by rapid cooling in diluted phenol
|
| Growth protocol |
Pseudomonas cells were grown in LB supplemented with 10mM MgCl2 at 37°C until OD600nm ̴ 0,3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were then lyzed in TRIzol,® total RNA was purified through a standard organic extraction and ethanol precipitation followed by DNase treatment (TURBO DNAse®) and rRNA depletion (Ribo-Zero rRNA Removal Kit)
RNA libraries were prepared for sequencing using standard Illumina protocols
Truseq stranded RNA seq kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Host RNA recovered after 13min post infection
Host-infect-P3_T0-T13_statistics.xls
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced were mapped to Pseudomonas aeruginosa PAK whole genome sequence or Phage P3 sequence complemented by the manually annotated ncRNA using bowtie v1.0.1 with parameter -k 2000
MAP2COV was used for counting genes with parameters -t RNA -b YES -l 20
Statistical analyses were performed using R version 3 and Bioconductor packages.
The whole dataset was first normalized using the normalization function of DESeq2 and with default parameters.
The statistical test function of DESeq2 was then applied on each strand coverage count with the 13M value as a reference for reporting the expression data of 0M.
Genome_build: Pseudomonas aeruginosa PAK (KK037225.1)
Supplementary_files_format_and_content: Genome annotation (Gene ID), Raw Counts (0M, 13M), Normalized Counts (0M, 13M), Statistics (FC, pvalue, significant)
|
| |
|
| Submission date |
Jan 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Monot Marc |
| E-mail(s) |
mmonot@pasteur.fr
|
| Phone |
+33145688390
|
| Organization name |
Institut Pasteur
|
| Department |
Genomes and Genetics
|
| Lab |
Biomics
|
| Street address |
25, rue du docteur roux
|
| City |
Paris |
| ZIP/Postal code |
75015 |
| Country |
France |
| |
|
| Platform ID |
GPL18644 |
| Series (1) |
| GSE76513 |
Biological characterization of virulent therapeutic bacteriophage PAK_P3 infecting Pseudomonas aeruginosa. |
|
| Relations |
| BioSample |
SAMN04383752 |
| SRA |
SRX1516058 |
