|
| Status |
Public on Jan 08, 2016 |
| Title |
KO_H2O_6624 |
| Sample type |
RNA |
| |
|
| Source name |
colon,Parp1-/-, H2O
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Colon
genotype: Parp1-/-
treatment: H2O
|
| Treatment protocol |
Mice were assigned to control group (continous access to plain drinking water) or to colitis group [4% dextran sulfate sodium (DSS) for 7 days]. At sacrifice, whole colon was colected, washed, and flash-frozen in liquuid N2 for RNA isolation.
|
| Growth protocol |
6-8 week old mice housed in conventional animal facility monitored and free of common murine pathogens (MHV, MPV, MVM, TMEV, Mycoplasma pulmonis, Sendai, EDIM, MNV, and eco- and endoparasites)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions, followed by column purification (Qiagen Mini RNA kit)
|
| Label |
biotin
|
| Label protocol |
According to menufacturer's (Affymetrix) protocol
|
| |
|
| Hybridization protocol |
According to menufacturer's (Affymetrix) protocol
|
| Scan protocol |
According to menufacturer's (Affymetrix) protocol
|
| Description |
Gene expression data from colon of Parp1-/- mice treated with H2O
|
| Data processing |
The data were analyzed with GeneSpring 13.1.1, using ExonRMA16 summarization algorithm; quantile sample normalization with baseline transformation to median of all samples
|
| |
|
| Submission date |
Jan 07, 2016 |
| Last update date |
Jan 08, 2016 |
| Contact name |
Pawel R. Kiela |
| E-mail(s) |
pkiela@peds.arizona.edu
|
| Phone |
520-626-9687
|
| Organization name |
University of Arizona
|
| Department |
Pediatrics
|
| Lab |
Gastroenterology and Nutrition
|
| Street address |
1501 N. Campbell Ave.
|
| City |
Tucson |
| State/province |
AZ |
| ZIP/Postal code |
85724 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE76658 |
Transcriptional reprogramming and resistance to colonic mucosal injury in PARP1-deficient mice |
|
