|
| Status |
Public on Sep 07, 2016 |
| Title |
TEM Sample 20 |
| Sample type |
RNA |
| |
|
| Source name |
Sorted PBMC (CD8+ TEM )
|
| Organism |
Macaca mulatta |
| Characteristics |
donorid: Rh20235
cell count: 259,000
timepoint: Pre Treatment
treatment: Anti-IL15 Ab
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using Agencourt RNAdvance Blood Kit. The isolated total RNA was checked for quantity and quality using a NanoDrop 2000c (Thermo Fisher Scientific) and an Experion Electrophoresis System. Samples with an RQI classification ≥7.0 were selected to proceed downstream to amplification. Samples were normalized at 50ng for input and amplified using Illumina TotalPrep RNA amplification kits (Ambion) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
The hybridized BeadChips were washed, blocked, stained and scanned according to the Illumina's direct hybridization protocol.
|
| |
|
| Hybridization protocol |
Microarray analysis was conducted using 750ng of biotinylated cRNA hybridized to Human RefSeq-12 V4 BeadChips (Illumina) at 58°C for 20 hours, according to Illumina's direct hybridization protocol.
|
| Scan protocol |
The arrays were scanned using an Illumina iScan scanner and quantified using Genome Studio software (Illumina).
|
| Data processing |
Raw probe intensities were preprocessed using the R statistical language. Missing values were imputed using the KNN algorithm followed by log2 transformation and quantile normalization using the Bioconductor software package limma.
|
| |
|
| Submission date |
Jan 12, 2016 |
| Last update date |
Sep 07, 2016 |
| Contact name |
AARTHI TALLA |
| E-mail(s) |
axt427@case.edu
|
| Phone |
4047695226
|
| Organization name |
Case Western Reserve University School of Medicine
|
| Department |
Pathology
|
| Lab |
Dr.Rafick Sekaly
|
| Street address |
2103 Cornell Rd
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE76796 |
Effect of anti-IL-15 administration on T cell and NK cell homeostasis in rhesus macaques (TEM) |
| GSE76797 |
Effect of anti-IL-15 administration on T cell and NK cell homeostasis in rhesus macaques |
|
