|
| Status |
Public on Feb 29, 2016 |
| Title |
G3BP1 Stress 3, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Human Brain
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U251-MG
cell type: astrocytoma
|
| Treatment protocol |
Cells were treated with 500uM aresenite to induce stress and RNA harvested after 30 minutes. Control cells (non stress) were treated with DMSO and RNA harvested from cells after 30 minutes.
|
| Growth protocol |
Cells were grown in DMEM media supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the RNeasy Mini Kit (Qiagen, Cat#74104 ) using the manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Human Exon 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400. CHECK WITH FACILITY
|
| Scan protocol |
GeneChips were scanned at The Centre for Applied Genomics (TCAG), SickKids Hospital Toronto, Canada
|
| Description |
RNA immunopreciptation (RIP) using G3BP1 antibody from cells treated with arsentite to induce stress granules. Biological replicate 3
|
| Data processing |
The data were normalized with Affymetrix Expression Console Software using RMA analysis.
|
| |
|
| Submission date |
Jan 13, 2016 |
| Last update date |
Feb 29, 2016 |
| Contact name |
James Rutka |
| Phone |
416-813-6425
|
| Organization name |
Hospital for Sick Children
|
| Department |
Cell Biology
|
| Street address |
686 Bay Street
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE76814 |
ECT2 and AURKB Modulate Formation of Stress Granules Containing Transcripts from Diverse Cellular Pathways in Astrocytoma Cells |
|
