|
| Status |
Public on Jan 20, 2016 |
| Title |
SJBALL21964 |
| Sample type |
RNA |
| |
|
| Source name |
Leukemia cells
|
| Organism |
Homo sapiens |
| Characteristics |
source: bone marrow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Hy5
|
| Label protocol |
miRCURY Power labeling kit used according to manufacturer's (Exiqon) instructions
|
| |
|
| Hybridization protocol |
hybridization was done at 56 degrees Celsius for 16 hours followed by washing on an HS Pro instrument from Tecan.
|
| Scan protocol |
Scanning was performed using an Agilent G2505B scanning system
|
| Description |
processed signal intensity
|
| Data processing |
raw counts were processed using the limma package(version 3.6.9) from Bioconductor
|
| |
|
| Submission date |
Jan 13, 2016 |
| Last update date |
Jan 20, 2016 |
| Contact name |
William Evans |
| E-mail(s) |
william.evans@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Pharmaceutical Sciences
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11434 |
| Series (1) |
|
