|
| Status |
Public on May 09, 2016 |
| Title |
Bach2-tdRFP low 1 |
| Sample type |
SRA |
| |
|
| Source name |
IgG1 LZ GC B cells, low Bach2-tdRFP
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: Bach2-tdRFP
tissue origination: spleen
cell type: NP-specific IgG1 light zone germinal center B cells
bach2-tdrfp expression: low
|
| Treatment protocol |
tdRFP low and high IgG1 LZ GC B cells were FACS-sorted from 3 Bach2-tdRFP mice and pooled for each sample.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
DNA libraries were prepared for sequencing using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
S06298
|
| Data processing |
Read alignment: TopHat2 v2.0.8 with default settings.
Expression level: Cufflinks v2.1.1 with default settings.
Genome_build: mm9 (MGSCv37)
Supplementary_files_format_and_content: *fpkm.txt: Includes FPKM values.
|
| |
|
| Submission date |
Jan 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
TOMOHIRO KUROSAKI |
| E-mail(s) |
kurosaki@ifrec.osaka-u.ac.jp
|
| Phone |
+81-6-6879-4458
|
| Organization name |
Osaka University Immunology Frontier Research Center
|
| Lab |
Laboratory of Lymphocyte Differentiation
|
| Street address |
3-1 Yamada-oka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE77319 |
Comparison of Bach2-tdRFP high expression and low expression NP-specific IgG1 light zone GC B cells |
|
| Relations |
| BioSample |
SAMN04445535 |
| SRA |
SRX1550094 |
