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| Status |
Public on May 09, 2016 |
| Title |
Comparison of Bach2-tdRFP high expression and low expression NP-specific IgG1 light zone GC B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Despite the importance of memory B cells for protection from recurrent infection, how these cells are selected during germinal center (GC) reactions remains unclear. We show here that light zone (LZ) GC B cells with lower affinity BCRs express a less CD40 signature and relatively high levels of Bach2, being prone to enter the memory B cell pool. We also find that Bach2 contributes to memory B cell generation in a Blimp-1-independent manner and that its higher expression confers on LZ GC cells a more advantage for entering the memory B cell compartment. Thus, our data support an instructive model in which weak T cell help keep Bach2 expression relatively high, thereby being predisposed to enter the memory pool.
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| Overall design |
mRNA expression profiles of Bach2-tdRFP low and high expression NP-specific IgG1 light zone GC B cells were generated by deep sequencing using Illumina HiSeq 1500.
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| Contributor(s) |
Kurosaki T, Shinnakasu R |
| Citation(s) |
27158841 |
| |
| Submission date |
Jan 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
TOMOHIRO KUROSAKI |
| E-mail(s) |
kurosaki@ifrec.osaka-u.ac.jp
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| Phone |
+81-6-6879-4458
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| Organization name |
Osaka University Immunology Frontier Research Center
|
| Lab |
Laboratory of Lymphocyte Differentiation
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| Street address |
3-1 Yamada-oka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA310018 |
| SRA |
SRP068978 |
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