|
Status |
Public on May 09, 2016 |
Title |
Bach2-tdRFP high 3 |
Sample type |
SRA |
|
|
Source name |
IgG1 LZ GC B cells, high Bach2-tdRFP
|
Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6 genotype/variation: Bach2-tdRFP tissue origination: spleen cell type: NP-specific IgG1 light zone germinal center B cells bach2-tdrfp expression: high
|
Treatment protocol |
tdRFP low and high IgG1 LZ GC B cells were FACS-sorted from 3 Bach2-tdRFP mice and pooled for each sample.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. DNA libraries were prepared for sequencing using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
|
|
Description |
S06305
|
Data processing |
Read alignment: TopHat2 v2.0.8 with default settings. Expression level: Cufflinks v2.1.1 with default settings. Genome_build: mm9 (MGSCv37) Supplementary_files_format_and_content: *fpkm.txt: Includes FPKM values.
|
|
|
Submission date |
Jan 27, 2016 |
Last update date |
May 15, 2019 |
Contact name |
TOMOHIRO KUROSAKI |
E-mail(s) |
kurosaki@ifrec.osaka-u.ac.jp
|
Phone |
+81-6-6879-4458
|
Organization name |
Osaka University Immunology Frontier Research Center
|
Lab |
Laboratory of Lymphocyte Differentiation
|
Street address |
3-1 Yamada-oka
|
City |
Suita |
State/province |
Osaka |
ZIP/Postal code |
565-0871 |
Country |
Japan |
|
|
Platform ID |
GPL18480 |
Series (1) |
GSE77319 |
Comparison of Bach2-tdRFP high expression and low expression NP-specific IgG1 light zone GC B cells |
|
Relations |
BioSample |
SAMN04445540 |
SRA |
SRX1550099 |