|
| Status |
Public on Dec 31, 2016 |
| Title |
PfAP2-I-GFP 42 hours post invasion |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
PfAP2-I-GFP RNA
|
| Organism |
Plasmodium falciparum |
| Characteristics |
strain: 3D7 PfAP2-I-GFP
hours post invasion: 42
|
| Growth protocol |
P. falciparum 3D7 with PfAP2-I C-terminal GFP tag and P. falciparum 3D7 with PfAP2-I C-terminal GFP tag and TGCA DNA motif mutated in msp5 promoter were grown under shaking conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Starting at 18 hours post-invasion, samples were harvested in Trizol for total RNA extraction every 6 hours until 36h post-invasion and then every 3h until the end of the IDC (48h post-invasion).
|
| Label |
Cy5
|
| Label protocol |
Painter, H. J., Altenhofen, L. M., Kafsack, B. F. C. & Llinás, M. Whole-genome analysis of Plasmodium spp. Utilizing a new agilent technologies DNA microarray platform. Methods Mol. Biol. 923, 213–219 (2013).
|
| |
|
| Channel 2 |
| Source name |
3D7 Mixed Stage Reference Pool
|
| Organism |
Plasmodium falciparum |
| Characteristics |
strain: 3D7
|
| Growth protocol |
P. falciparum 3D7 with PfAP2-I C-terminal GFP tag and P. falciparum 3D7 with PfAP2-I C-terminal GFP tag and TGCA DNA motif mutated in msp5 promoter were grown under shaking conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Starting at 18 hours post-invasion, samples were harvested in Trizol for total RNA extraction every 6 hours until 36h post-invasion and then every 3h until the end of the IDC (48h post-invasion).
|
| Label |
Cy3
|
| Label protocol |
Painter, H. J., Altenhofen, L. M., Kafsack, B. F. C. & Llinás, M. Whole-genome analysis of Plasmodium spp. Utilizing a new agilent technologies DNA microarray platform. Methods Mol. Biol. 923, 213–219 (2013).
|
| |
|
| |
| Hybridization protocol |
Painter, H. J., Altenhofen, L. M., Kafsack, B. F. C. & Llinás, M. Whole-genome analysis of Plasmodium spp. Utilizing a new agilent technologies DNA microarray platform. Methods Mol. Biol. 923, 213–219 (2013).
|
| Scan protocol |
Agilent G2600D Microarray Scanner. Images were quantified using Agilent Feature Extraction Software (version 11.5.1.1).
|
| Description |
3D7 gene PfAP2-I with C-terminalGFP
42 hpi wt
|
| Data processing |
Agilent Feature Extraction Software (v 11.5.1.1) was used for background subtraction.
|
| |
|
| Submission date |
Feb 10, 2016 |
| Last update date |
Dec 31, 2016 |
| Contact name |
Manuel Llinas |
| E-mail(s) |
manuel@psu.edu
|
| Phone |
8148673444
|
| Organization name |
Penn State University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Manuel Llinas Lab
|
| Street address |
Millennium Science Complex, Pollock Rd
|
| City |
University Park |
| State/province |
Pennsylvania |
| ZIP/Postal code |
16802 |
| Country |
USA |
| |
|
| Platform ID |
GPL15130 |
| Series (2) |
| GSE77807 |
PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7 [timecourse 1] |
| GSE77810 |
PfAP2-I-GFP-msp5MUT vs PfAP2-I-GFP 3D7 |
|
