|
| Status |
Public on Sep 13, 2016 |
| Title |
LP-1/Cfz_rep2_261889 |
| Sample type |
RNA |
| |
|
| Source name |
LP-1/Cfz multiple myeloma cells resistant to 12 nM carfilzomib
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LP-1/Cfz
|
| Treatment protocol |
LP-1/Cfz cells were profiled for gene expression after 1 week of growth in the absence of carfilzomib together with parental LP-1 cells which had not been selected in the drug.
|
| Growth protocol |
Cells for gene expression profiling were cultured in RPMI 1640 with GlutaMAX (Life Technologies) supplemented with 4% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with the miRNeasy mini kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared by Expression Analysis (Durham, NC).
|
| |
|
| Hybridization protocol |
Hybridization of cRNA on Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays was performed by Expression Analysis.
|
| Scan protocol |
Scanning of GeneChip arrays was performed by Expression Analysis.
|
| Description |
Gene expression data from LP-1/Cfz multiple myeloma cells resistant to 12 nM carfilzomib
SAMPLE 1.2
|
| Data processing |
The data were analyzed with GeneSpring software (Agilent Technologies) using default settings and the RMA normalization and summarization algorithm.
|
| |
|
| Submission date |
Feb 18, 2016 |
| Last update date |
Sep 14, 2016 |
| Contact name |
Robert Hawley |
| Organization name |
George Washington University
|
| Department |
Anatomy and Regenerative Biology
|
| Street address |
2300 I Street NW, Suite 431
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20037 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE78069 |
Comparative expression analysis of carfilzomib-resistant and parental LP-1 human multiple myeloma cells |
|
