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Status |
Public on Sep 13, 2016 |
Title |
LP-1_rep2_261884 |
Sample type |
RNA |
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|
Source name |
LP-1 multiple myeloma cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: LP-1
|
Treatment protocol |
LP-1/Cfz cells were profiled for gene expression after 1 week of growth in the absence of carfilzomib together with parental LP-1 cells which had not been selected in the drug.
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Growth protocol |
Cells for gene expression profiling were cultured in RPMI 1640 with GlutaMAX (Life Technologies) supplemented with 4% fetal bovine serum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with the miRNeasy mini kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared by Expression Analysis (Durham, NC).
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Hybridization protocol |
Hybridization of cRNA on Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays was performed by Expression Analysis.
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Scan protocol |
Scanning of GeneChip arrays was performed by Expression Analysis.
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Description |
Gene expression data from LP-1 multiple myeloma cells SAMPLE 2.2
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Data processing |
The data were analyzed with GeneSpring software (Agilent Technologies) using default settings and the RMA normalization and summarization algorithm.
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Submission date |
Feb 18, 2016 |
Last update date |
Sep 14, 2016 |
Contact name |
Robert Hawley |
Organization name |
George Washington University
|
Department |
Anatomy and Regenerative Biology
|
Street address |
2300 I Street NW, Suite 431
|
City |
Washington |
State/province |
DC |
ZIP/Postal code |
20037 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE78069 |
Comparative expression analysis of carfilzomib-resistant and parental LP-1 human multiple myeloma cells |
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