|
| Status |
Public on Mar 04, 2016 |
| Title |
MG1363_Glu_10min_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
MG1363, glucose depleted, sampled 10 min after depletion
|
| Organism |
Lactococcus cremoris subsp. cremoris MG1363 |
| Characteristics |
strain: MG1363
|
| Treatment protocol |
Growth was stopped immidiately by transfer of 10 ml culture sample into a thick glass flask precooled on ice followed by rapid swirling
|
| Growth protocol |
Exponentially growing strains in SA medium with 1% glucose were diluted 100-fold in preformed SA medium with 3 mM glucose. After six generations of exponential growth the culture was diluted 100-fold into prewarmed SA medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were pelleted by centrifulation and washed in ice cold saline water (0.9% NaCl). RNA was extracted by the hot phenol method
|
| Label |
Cy3
|
| Label protocol |
Labelling according to Agilent protocol using random hexamer and Cy3
|
| |
|
| Hybridization protocol |
Hybridization according to Agilent protocol. Agilent 8x15k custom microarray G2509F
|
| Scan protocol |
Agilent Technologies Scanner G2505C. Agilent's Feature Extraction software.
|
| Data processing |
Quantile normalization (R Bioconductor package preprocessCore)
|
| |
|
| Submission date |
Mar 03, 2016 |
| Last update date |
Mar 04, 2016 |
| Contact name |
Mogens Kilstrup |
| E-mail(s) |
mki@bio.dtu.dk
|
| Phone |
+45 25538815
|
| Organization name |
Technical University of Denmark
|
| Department |
DTU Systems Biology
|
| Lab |
Metabolic Signaling and Regulation
|
| Street address |
Building 301 room 158
|
| City |
Kgs. Lyngby |
| ZIP/Postal code |
DK2800 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL21561 |
| Series (1) |
| GSE78882 |
Gene expression signature after abrupt glucose starvation in Lactococcus lactis |
|
