 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 15, 2016 |
| Title |
Cumulus cells (2 month-old), strain: C57BL/6 1 |
| Sample type |
SRA |
| |
|
| Source name |
Cumulus cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: 2 months
|
| Growth protocol |
Minimum Essential Medium alpha (MEMα) with 75 μg/ml penicillin G, 50 μg/ml streptomycin sulfate, 0.23 mM pyruvate, 3 mg/ml bovine serum albumin (BSA; Sigma-Aldrich, St Louis, MO), and 10 μM of the phosphodiesterase inhibitor, milrinone (Sigma-Aldrich) was used as the basic culture medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a miRCURY RNA Isolation Kit
Libraries for the RNA-seq were prepared from total RNAs extracted from the MGCs of eCG-primed mice according to the manufacturer's protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
Shiroguchi_Index04
|
| Data processing |
Basecalls performed using Illumina offline basecaller (OLB) version 1.9.4
Sequences were aligned on mouse genome (mm9) using tophat2 (version 2.1.0) with option "--no-coverage search".
Gene expression was estimated using cufflinks (version 2.1.0) with default options.
Genome_build: mm9
Supplementary_files_format_and_content: plain text containig FPKM (fragments per kilobase exon per million reads) values.
|
| |
|
| Submission date |
Apr 15, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Takaho A. Endo |
| E-mail(s) |
takaho.endo@riken.jp
|
| Organization name |
RIKEN
|
| Department |
IMS
|
| Lab |
Laboratory for Integrative Genomics
|
| Street address |
1-7-22 Suehiro, Tsurumi
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE80326 |
Assessing transcriptomes of mouse cumulus cells, mural granulosa cells, and cumulus-oocyte complexes |
|
| Relations |
| BioSample |
SAMN04858529 |
| SRA |
SRX1707234 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
