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GEO help: Mouse over screen elements for information. |
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Status |
Public on Apr 15, 2016 |
Title |
Cumulus cells (2 month-old), strain: C57BL/6 1 |
Sample type |
SRA |
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Source name |
Cumulus cells
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Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 age: 2 months
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Growth protocol |
Minimum Essential Medium alpha (MEMα) with 75 μg/ml penicillin G, 50 μg/ml streptomycin sulfate, 0.23 mM pyruvate, 3 mg/ml bovine serum albumin (BSA; Sigma-Aldrich, St Louis, MO), and 10 μM of the phosphodiesterase inhibitor, milrinone (Sigma-Aldrich) was used as the basic culture medium.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using a miRCURY RNA Isolation Kit Libraries for the RNA-seq were prepared from total RNAs extracted from the MGCs of eCG-primed mice according to the manufacturer's protocol
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 1500 |
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Description |
Shiroguchi_Index04
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Data processing |
Basecalls performed using Illumina offline basecaller (OLB) version 1.9.4 Sequences were aligned on mouse genome (mm9) using tophat2 (version 2.1.0) with option "--no-coverage search". Gene expression was estimated using cufflinks (version 2.1.0) with default options. Genome_build: mm9 Supplementary_files_format_and_content: plain text containig FPKM (fragments per kilobase exon per million reads) values.
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Submission date |
Apr 15, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Takaho A. Endo |
E-mail(s) |
takaho.endo@riken.jp
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Organization name |
RIKEN
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Department |
IMS
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Lab |
Laboratory for Integrative Genomics
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Street address |
1-7-22 Suehiro, Tsurumi
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City |
Yokohama |
State/province |
Kanagawa |
ZIP/Postal code |
230-0045 |
Country |
Japan |
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Platform ID |
GPL18480 |
Series (1) |
GSE80326 |
Assessing transcriptomes of mouse cumulus cells, mural granulosa cells, and cumulus-oocyte complexes |
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Relations |
BioSample |
SAMN04858529 |
SRA |
SRX1707234 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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