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Status |
Public on Jun 03, 2016 |
Title |
skin_noUv_WT_4_mRNA |
Sample type |
RNA |
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Source name |
dorsal skin, not irradiated, WT(Ppard+/+)
|
Organism |
Mus musculus |
Characteristics |
background strain: SKH1-C57/Bl6-SV129 tissue: skin uv irradiation: no_UV genotype: WT(Ppard+/+)
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Treatment protocol |
Irradiated mice: 70mJ/cm2 of UVB three times a week
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Growth protocol |
Mice in standard colony, with water and food ad libitum
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA containing the small RNA fraction was isolated using TRIzol reagent (Invitrogen).
|
Label |
biotin
|
Label protocol |
For each sample, 100ng of total RNA were amplified using the Affymetrix Whole-Transcript (WT) Sense Taget Labeling protocol. 5.5μg of the cDNA was fragmented and labeled with GeneChip® WT Terminal Labeling kit (901525, Affymetrix).
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Hybridization protocol |
Affymetrix mouse gene 1.0ST arrays (Affymetrix, Santa Clara, CA, USA) were hybridized with 2.3μg of fragmented target, at 45°C for 16 hours washed and stained according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual (Fluidics protocol FS450_0007).
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Scan protocol |
The arrays were scanned with the GeneChip® Scanner 3000 7G (Affymetrix).
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Description |
wt592
|
Data processing |
Background subtraction, quantile normalization and probeset summarization were performed with the Affymetrix Power Tools command line tool, using the RMA method.
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Submission date |
Apr 19, 2016 |
Last update date |
Jun 03, 2016 |
Contact name |
Mark Ibberson |
Organization name |
SIB Swiss Institute of Bioinformatics
|
Department |
Vital-IT
|
Street address |
Genopode building
|
City |
Lausanne |
ZIP/Postal code |
CH-1015 |
Country |
Switzerland |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE80427 |
Identification of a novel PPARβ/δ / miR-21-3p axis in UV-induced skin inflammation [mouse mRNA] |
GSE80431 |
Identification of a novel PPARβ/δ / miR-21-3p axis in UV-induced skin inflammation |
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