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| Status |
Public on Jun 03, 2016 |
| Title |
Identification of a novel PPARβ/δ / miR-21-3p axis in UV-induced skin inflammation [mouse mRNA] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Although excessive exposure to UV is widely recognized as a major factor leading to skin perturbations and cancer, the complex mechanisms underlying inflammatory skin disorders resulting from UV exposure remain incompletely characterized. The nuclear hormone receptor PPARβ/δ is known to control cutaneous repair and UV-induced cancer development. Here, we describe a novel PPARβ/δ-dependent molecular cascade involving TGFβ-1 and miR-21-3p, which is activated in the epidermis in response to UV exposure. We establish that the passenger miRNA miR-21-3p, that we identify as a novel UV-induced miRNA in the epidermis, plays a pro-inflammatory function in keratinocytes, and that its high level of expression in human skin is associated with psoriasis and squamous cell carcinomas. Finally, we provide evidence that inhibition of miR-21-3p reduces UV-induced cutaneous inflammation in ex vivo human skin biopsies, thereby underlining the clinical relevance of miRNA-based topical therapies for cutaneous disorders.
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| Overall design |
16 samples: 4 KO (Ppard-/-) and 4 WT (Ppard+/+) irradiated for 12 weeks; 4 KO and 4 WT without irradiation
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| Contributor(s) |
Michalik L, Degueurce G |
| Citation(s) |
27250636 |
| |
| Submission date |
Apr 19, 2016 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Mark Ibberson |
| Organization name |
SIB Swiss Institute of Bioinformatics
|
| Department |
Vital-IT
|
| Street address |
Genopode building
|
| City |
Lausanne |
| ZIP/Postal code |
CH-1015 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE80431 |
Identification of a novel PPARβ/δ / miR-21-3p axis in UV-induced skin inflammation |
|
| Relations |
| BioProject |
PRJNA318895 |
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