|
Status |
Public on Jun 03, 2016 |
Title |
Identification of a novel PPARβ/δ / miR-21-3p axis in UV-induced skin inflammation [mouse mRNA] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
Although excessive exposure to UV is widely recognized as a major factor leading to skin perturbations and cancer, the complex mechanisms underlying inflammatory skin disorders resulting from UV exposure remain incompletely characterized. The nuclear hormone receptor PPARβ/δ is known to control cutaneous repair and UV-induced cancer development. Here, we describe a novel PPARβ/δ-dependent molecular cascade involving TGFβ-1 and miR-21-3p, which is activated in the epidermis in response to UV exposure. We establish that the passenger miRNA miR-21-3p, that we identify as a novel UV-induced miRNA in the epidermis, plays a pro-inflammatory function in keratinocytes, and that its high level of expression in human skin is associated with psoriasis and squamous cell carcinomas. Finally, we provide evidence that inhibition of miR-21-3p reduces UV-induced cutaneous inflammation in ex vivo human skin biopsies, thereby underlining the clinical relevance of miRNA-based topical therapies for cutaneous disorders.
|
|
|
Overall design |
16 samples: 4 KO (Ppard-/-) and 4 WT (Ppard+/+) irradiated for 12 weeks; 4 KO and 4 WT without irradiation
|
|
|
Contributor(s) |
Michalik L, Degueurce G |
Citation(s) |
27250636 |
|
Submission date |
Apr 19, 2016 |
Last update date |
Mar 04, 2019 |
Contact name |
Mark Ibberson |
Organization name |
SIB Swiss Institute of Bioinformatics
|
Department |
Vital-IT
|
Street address |
Genopode building
|
City |
Lausanne |
ZIP/Postal code |
CH-1015 |
Country |
Switzerland |
|
|
Platforms (1) |
GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
Samples (16)
|
|
This SubSeries is part of SuperSeries: |
GSE80431 |
Identification of a novel PPARβ/δ / miR-21-3p axis in UV-induced skin inflammation |
|
Relations |
BioProject |
PRJNA318895 |