|
| Status |
Public on Jan 01, 2017 |
| Title |
2H 128μg/ml GR-SU_vs_control_1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
S.aureus 2H 128μg/ml GR-SU
|
| Organism |
Staphylococcus aureus subsp. aureus MW2 |
| Characteristics |
strain: MW2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the bacterial cells using a FastRNA Pro Blue Kit (MP Biomedicals, Cleveland, OH, USA) according to the manufacturer’s protocol.
|
| Label |
Cy3
|
| Label protocol |
Test and control cDNAs were labelled with Cy3 and Cy5 (GE Healthcare UK), respectively.
|
| |
|
| Channel 2 |
| Source name |
S.aureus 2H 0μg/ml GR-SU
|
| Organism |
Staphylococcus aureus subsp. aureus MW2 |
| Characteristics |
strain: MW2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the bacterial cells using a FastRNA Pro Blue Kit (MP Biomedicals, Cleveland, OH, USA) according to the manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
Test and control cDNAs were labelled with Cy3 and Cy5 (GE Healthcare UK), respectively.
|
| |
|
| |
| Hybridization protocol |
65℃ 20H
|
| Scan protocol |
The arrays were then scanned with an Agilent scanner (Agilent Technologies).
|
| Description |
Gene expression after 2H
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Apr 20, 2016 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Kentaro Oyama |
| Organization name |
Department of Oral Microbiology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
|
| Street address |
Sakuragaoka 8-35-1
|
| City |
Kagoshima City |
| ZIP/Postal code |
890-8544 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21747 |
| Series (1) |
| GSE80500 |
Effect of Glycyrrhetinic acid and its derivatives on antibacterial activity and virulence against Staphylococcus aureus |
|
